Bruce McEwen Laboratory
Selected Publications
McEwen, B.F., Barnard, R.M., Portuese, T., and Hsieh, C-E. (2002) Using electron tomography to determine the interaction between mammalian kinetochores and microtubules. Proc. Int. Congress Elect. Microsc. 15, Durban, South Africa, 1-6 September, 2002 P179-180. - First publication on detection of plus-end conformations for kinetochore microtubules.
McEwen, B.F., Marko, M., Hsieh, C-E., and Mannella, C.A., Use of Frozen-hydrated Axonemes to Assess Imaging Parameters and Resolution Limits in Cryo-Electron Tomography. J. Struct. Biol. 2002 138, 47-57. - Electron tomography on a frozen-hydrated preparation of a microtubule-based motility organelle; includes a through examination of factors determining resolution in electron tomography. This paper was cited in Science in a review of cryo-electron tomography as one of the breakthroughs for 2002 (Science 298 20 Dec.2002 - on line extras).
McEwen, B.F., Chan, G.K.T., Zubrowski, B., Savoian, M.S., Sauer, M.T., and Yen, T. (2001). CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells. Mole. Biol. Cell 12, 2776-2789. - Initial characterization of the structural and functional consequences of knocking out a critical kinetochore component.
McEwen, B.F., and Frank, J. Electron tomographic and other approaches for imaging molecular machines. Current Opinion in Neurobiology 11 594-600, 2001. - Invited review emphasizing the interface between electron tomography and high resolution methods.
McEwen, B.F., and Marko, M. (2001) The emergence of electron tomography as an important tool for investigating cellular ultrastructure. J. Histochem. Cytochem. 49, 553-563. - Invited review on electron tomography; includes a summary of recent applications.
Howell, B.J., Canman, J.C., McEwen, B.F., Rieder, C.L., Hoffman, D.B., and Salmon, E.D. (2001). Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation. J. Cell Biol. 155, 1159-1172
McEwen, B.F., and Marko, M. (1999) Three-dimension transmission electron microscopy and its application to mitosis research. Methods in Cell Biology: Mitosis and Meiosis. 61, 81-111. - Invited methods paper: describes technical details of current methods for alignment and segmentation in electron tomography.
McEwen, B.F., Hsieh, C-E., Mattheyses, A.L. and Rieder, C.L. (1998). A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitution. Chromosoma 107: 366-375. - Demonstrates the importance of using freeze-fixation when working with the kinetochore. This publication won the Chromosoma Prize for best paper of the year in the journal.
McEwen, B.F., Ding, Ye, and Heagle, Amy B. (1998). Relevance of kinetochore size and microtubule-binding capacity for stable chromosome attachment during mitosis in PtK1 cells. Chromosome Research 6, 123-132.
McEwen, B.F., and Heagle, A.B. (1997) Electron microscopic tomography: a tool for probing the structure and function of subcellular components. J. Imaging Syst. Technol. 8, 175-187. - Invited contribution: reviews basic principles of electron tomography and presents first data on kinetochore/microtubule interaction.
McEwen, B.F., Heagle, A.B.,Cassels, G.O., Buttle, K.F., and Rieder, C.L. (1997). Kinetochore-fiber maturation in PtK1 cells and its implications for the mechanisms of chromosome congression and anaphase onset. J. Cell Biol. 137, 1567-1580. This paper documents the development of the kinetochore fiber through different stages of mitosis and in response to taxol treatment. It also demonstrates that the direction of chromosome motion during congression is not dependent upon the number of microtubules attached to each sister kinetochore.
Khodjakov, A., Cole, R.W., McEwen, B.F., Buttle, K.F., and Rieder, C.L. (1997) Chromosome fragments possessing only one kinetochore can congress to the spindle equator. J.Cell Biol. 136, 229-240.
Landis, W.J., Hodgens, K.J, Song, M.J., Arena, J., Kiyonaga, S., Marko, M., Owen, C., and McEwen, B.F. Mineralization of collagen may occur on fibril surfaces: Evidence from conventional and high-voltage electron microscopy and three-dimensional imaging. J. Structural Biology. 117 (1996) 24-35.
McEwen, B.F., Downing, K.H., and Glaeser, R.M. (1995). The relevance of dose-fractionation in tomography of radiation-sensitive specimens. Ultramicroscopy 60, 357-373. - A study of the relation between angular sampling and statistical significance in electron tomography.
McEwen, B.F., Arena, J., Frank, J. and Rieder, C.L. (1993) Structure of the colcemid-treated PtK1 kinetochore outer plate as determined by high voltage electron microscopic tomography. J. Cell Biol. 120: 301-312. - First application of electron tomography to mammalian kinetochores.
Landis, W.J., Song, M.J., Leith, A., McEwen, L., and McEwen, B.F. Mineral and organic matrix interaction in normally calcifying tendon visualized in three dimensions by high-voltage electron microscopic tomography and graphic image reconstruction. J.Structural Biology. 110 (1993) 39-54.
Barnard, D.P., Turner, J.N., Frank, J., and McEwen, B.F. (1992) A 360o single axis tilt-stage for the high-voltage electron microscope. J. Microsc. 167, 39-48. - Describes a tilt stage capable of complete angular coverage for specimens that can be prepared in a cylindrical geometry.
McEwen, B.F., Radermacher, M., Rieder, C.L., and Frank, J. (1986) Tomographic three-dimensional reconstruction of cilia ultrastructure from thick sections. Proc. Natl. Acad. Sci., U.S.A. 83, 9040-9044. - The first tomographic reconstruction of a well-studied test object that could be used to assess the effectiveness of electron tomography.