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DIETARY RESTRICTION PROTECTS MITOCHONDRIA AGAINST PERMEABILITY TRANSITION B.F. Krasnikov
and B.S. Kristal
The ability of dietary restricted (DR) animals to live longer and to be more resistant to different injuries compared to ad libitum (AL) animals has focused biomedical research over the last decades in attempt to understand some of the physiological and biochemical mechanisms of this phenomena. As shown by many investigators, dietary restriction can beneficially effect mitochondrial structure and function. Evidence of mitochondrial participation in apoptosis and aging processes has led to further study of mitochondrial function in DR animals. In the present study, we examined calcium induced permeability transition (PT) in liver mitochondria from 18 month AL and DR F344X Brown Norway rats in three different buffers in both the presence and absence of inorganic phosphate. Four parameters: oxygen uptake, mitochondrial membrane potential, calcium fluxes and swelling were recorded simultaneously in a 1ml-closed chamber. DR mitochondria were more resistant to PT induction then AL in all tested conditions.
For further information contact...Carmen Mannella: carmen@wadsworth.org |
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