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Cellular Immunology Proficiency Testing Program

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Links to CLEP

Lymphoid/T-Lymphocyte Immunophenotyping

In addition to the references listed below, we suggest consulting the following protocols that can be found in Current Protocols in Cytometry, published by John Wiley & Sons (2003):

  • Unit 6.5: Immunophenotypic Analysis of Peripheral Blood Lymphocytes
  • Unit 6.8: Enumeration of Absolute Cell Counts Using Immunophenotypic Techniques

Guidelines

  • Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes; Approved Guideline. NCCLS Vol. 18(21), H42-A. 1998.
  • Guidelines for performing single-platform absolute CD4+ T-cell determinations with CD45 gating for persons infected with human immunodeficiency virus. MMWR Vol. 52/No. RR-2.
  • 1997 Revised Guidelines for the Performance of CD4+ T-cell Determinations in Persons Infected with Human Immunodeficiency Virus (HIV). MMWR Vol. 46/No. RR-2.
  • 1994 Revised Guidelines for the Performance of CD4+ T-cell Determinations in Persons with Human Immunodeficiency Virus (HIV) Infection. MMWR Vol. 43/No. RR-3.

Journal References

Bergeron, M., J. K. Nicholson, S. Phaneuf, T. Ding, N. Soucy, A. D. Bradley, N. C. H. Foss, and F. Mandy. 2002. Selection of lymphocyte gating protocol has an impact on the level of reliability of T-cell subsets in aging specimens. Cytometry (Communications in Clinical Cytometry) 50:53-61.

Kutok, J. L., A. O. Roma, S. J. Lemire, and D. M. Dorfman. 1998. Four-color flow cytometric immunophenotypic determination of peripheral blood CD4+ T-lymphocyte counts: a comparison of validity and cost-effectiveness with a two-color method. American Journal of Clinical Pathology 110:465-70.

Nicholson, J. K. A., M. Hubbard, and B. M. Jones. 1996. Use of CD45 Fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry. Cytometry (Communications in Clinical Cytometry) 26:16-21.

O'Gorman, Maurice R.G. and Nicholson, Janet K.A. 2000. Adoption of single- platform technologies for enumeration of absolute T-lymphocyte subsets in peripheral blood. Clinical and Diagnostic Laboratory Immunology 7:333-335.

Xavier, B., G.E. Marti, and T.A. Fleisher. 1997. Comparative Analysis of Whole Blood Lysis Methods for Flow Cytometry. Cytometry (Communications in Clinical Cytometry) 30:1124-133.