Skip header information|
Wadsworth Center Home - Science in the Pursuit of Health|
Main Body

Cellular Immunology Proficiency Testing Program

Welcome

Links to CLEP

Non-lymphoid Immunophenotyping

In addition to the references listed below, we suggest consulting the following protocols that can be found in Current Protocols in Cytometry, published by John Wiley & Sons (2003):

  • Unit 6.4: Enumeration of CD34+ Hematopoietic Stem & Progenitor Cells
  • Unit 6.11: Immunophenotypic Analysis of PNH Cells

Journal References

CD34 Cell Enumeration

Arseniev, L., N. Pickerd, L. Goudeva, B. Hertenstein, and A. Ganser. 1999. Comparative evaluation of commonly used clones and fluorochrome conjugates of monoclonal antibodies for CD34 antigen detection. Journal of Hematotherapy and Stem Cell Research 8:547-559.

Barbosa, I.L., M.E. Sousa, M.I.. Godinho, F. Sousa, and A. Carvalhais. 1999. Single- versus dual-platform assays for human CD34+ cell enumeration. Cytometry (Communications in Clinical Cytometry) 38:274-279.

Barnett, D., G. Janossy, A. Lubenko, E. Matutes, A. Newland, and J.T. Reilly. 1999. Guideline for the flow cytometric enumeration of CD34+ haematopoietic stem cells. Clinical Lab Haematology 21:301-308.

Brocklebank, A.M., and R.L. Sparrow. 2001. Enumeration of CD34+ cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy. Cytometry (Communications in Clinical Cytometry) 46:254-261.

Brando, B., J. Wolfgang Gohde, B. Scarpati, and G. D'Avanzo. 2001. The "Vanishing Counting Bead" phenomenon: effect on absolute CD34+ cell counting in phosphate-buffered saline-diluted leukapheresis samples. Cytometry 43:154-160.

Gratama, J.W., D.R. Sutherland, and M. Keeney. 2001. Flow Cytometric Enumeration and Immunophenotyping of Hematopoietic Stem and Progenitor Cells. Seminars in Hematology 38:139-147.

Greco, N.J. et al.. 2003. Characterization of mulitple CD34+ cell populations in cord blood. Journal of Hematotherapy & Stem Cell Research 12:199-213.

Keeney, M., I. Chin-Yee, K. Weir, J. Popma, R. Nayar, and D. R. Sutherland. 1998. Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. Cytometry (Communications in Clinical Cytometry) 34:61-70.

Law, P., L. Traylor, and D.J. Recktenwald. 1999. Cell Analysis for Hematopietic Stem/Progenitor Cell Transplantation. Cytometry (Communications in Clinical Cytometry) 38:47-52.

Manion, K., and T. Frey. 1996. Apoptosis of cells in aged samples as detected by the ProCOUNT reagent. Cytometry (Communications in Clinical Cytometry) 26:317-322.

Marti, G., H. Johnsen, R. Sutherland, and S. Serke. 1998. A convergence of methods for a worldwide standard for CD34+ cell enumeration. Journal of Hematotherapy 7:105-109.

Piedras-Ross, J., E. Leon-Rodriguez, S. Sanchez-Guerrero, and X. Lopez-Karpovitch. 2003. Comparison of single- and dual-platform approaches to enumerate CD34+ cells in bone marrow and mobilized peripheral blood stem cells. Archives of Medical Research 34:16-19.

Leukocyte Adhesion Deficiency (LAD)

Andrian, U. H. v., et al. 1993. In vivo behavior of neutrophils from two patients with distinct inherited adhesion deficiency syndromes. Journal of Clinical Investigation 91:2893-2897.

Etzioni, A. 1996. Adhesion molecules: their role in health and disease. Pediatric Research 39:191-198.

O'Gorman, M. R.G., A.C. McNally, D.C. Anderson, and B.L. Moyones. 1993. A rapid whole blood lysis technique for the diagnosis of moderate or severe leukocyte adhesion deficiency (LAD). Annals of the New York Academy of Sciences 677:427-430.

Phillips, M.L., B.R. Schwartz, A. Etzioni, R. Bayer, H.D. Ochs, J.C. Paulson, and J.M. Harlan. 1995. Neutrophil adhesion in leukocyte adhesion deficiency syndrome type 2. Journal of Clinical Investigations 96:2898-2906.

Paroxysmal Nocturnal Hemoglobinuria (PNH)

Brodsky, R.A., G.L. Mukhina, S. Li, K.L. Nelson, P.L. Chiurazzi, J. T. Buckley, M.J. Borowitz. 2000. Improved detection and characterization of paroxysmal nocturnal hemoglobinuria using fluorescent aerolysin. Hematopathology 114:459-466.

Hall, S.E., and W.F. Rosse. 1996. The use of Monoclonal Antibodies and Flow Cytometry in the Diagnosis of Paroxysmal Nocturnal Hemoglobinuria. Blood 87:5332-5340.

Hernandez-Campo, P. M., M. Martin-Ayuso, J. Almeida, A. Lopez, and A. Orfao. 2002. Comparative analysis of different flow cytometry-based immunophenotypic methods for the analysis of CD59 and CD55 expression on major peripheral blood cell subsets. Cytometry (Clinical Cytometry) 50:191-201.

Oelschlaegel, U., I. Besson, C. Arnoulet, D. Sainty, R. Nowak, R. Naumann, Y. Bux, and G. Ehninger. 2001. A standardized flow cytometric method for screening paroxysmal nocturnal haemoglobinuria (PNH) measuring CD55 and CD59 expression on erythrocytes and granulocytes. Clinical Laboratory Haematology 23:81-90.

Richards, S.J., A.C. Rawstron, and P. Hillmen. 2000. Application of Flow Cytometry to the Diagnosis of Paroxysmal Nocturnal Hemoglobinuria. Cytometry (Communications in Clinical Cytometry) 42:223-233.