Slide 040 Basophil

Basophil and Metamyelocyte

image, description below
This image was taken from the March 2001 Cytohematology Proficiency Test Event - Slide 040. Basophils are granulocytes that contain purple-blue granules that contain heparin and vasoactive compounds. They comprise approximately 0.5% of the total leukocyte count. Basophils are the smallest circulating granulocytes, averaging 10 to 15 Ám in diameter. The nucleus to cytoplasm ratio is about 1:1, and the nucleus is often unsegmented or bilobed, rarely with three or four lobes. The chromatin pattern is coarse and patchy, staining a deep blue to reddish-purple. The cytoplasm is a homogenous pale blue, but this is often obscured by the large dark granules. Metamyelocytes have a cell diameter from 10 to 18 µm, and the nucleus to cytoplasm ratio is 1:1. Indentation of the nucleus begins at this stage, forming an outline that varies from slightly kidney shaped, to that of a broad V shape.

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