Clinical Chemistry Method Principle Codes

A. Hexokinase, UV

B. Hexokinase, colorimetric

C. Glucose oxidase, colorimetric

D. Glucose oxidase, O₂ electrode

E. o-Toluidine

F. Glucose dehydrogenase

G. Oxygen rate

Z. Other

UREA NITROGEN

A. Urease with glutamate dehydrogenase

B. Diacetyl monoxime

C. Urease, conductivity rate

D. Urease with indicator dye

E. Urease/hypochlorite (Berthelot)

F. Urease/Nessler's

G. Urease/Ammonium ion selective electrode

H. o-Phthalaldehyde

Z. Other

CREATININE

A. Alkaline picrate - Jaffe (kinetic/rate)

B. Alkaline picrate - Jaffe (endpoint)

C. Alkaline picrate - Jaffe (w/Lloyd's reagent)

D. Enzymatic (creatinine iminohydrolase)

E. Enzymatic (creatinine amidohydrolase)

Z. Other

URIC ACID

A. Uricase (NAD-NADH reaction)

B. Uricase/allantoin (differential absorption)

C. Uricase/peroxidase (colorimetric)

D. Phosphotungstic acid (Caraway-Henry)

Z. Other

BILIRUBIN (TOTAL)

A. Diazotized sulfanilic acid with blank (Jendrassik-Gróf)

B. Diazotized sulfanilic acid without blank (Jendrassik-Gróf)

C. Diazotized sulfanilic acid (Malloy-Evelyn)

D. Enzymatic (bilirubin oxidase)

E. Azobilirubin/dyphylline

F. Diazonium salt (DPD, DCAD, etc.)

Z. Other

PHOSPHORUS

A. Phosphomolybdate-no reduction

B. Phosphomolybdate reduction (ANS)

C. Phosphomolybdate reduction (stannous chloride)

D. Phosphomolybdate reduction (PMA phenol)

E. Phosphomolybdate reduction (ascorbic acid/malonic acid

F. Phosphomolybdate reduction-other

Z. Other

CALCIUM

A. o-Cresolphthalein

B. o-Cresolphthalein (with dialysis)

C. Atomic Absorption

D. Arsenazo dye

E. Alizarin/methylthymol blue complex

Z. Other

MAGNESIUM

A. Calmagite

B. Methylthymol Blue

C. Atomic Absorption

D. Xylidyl blue (Magon)

E. Formazon dye

F. Chlorophosphonazo III

Z. Other

IRON

A. Bathophenantroline

B. Ferrachrome/Ferrozine iron reagent

C. Tripyridyltriazine (TPZ)

D. Coulometric

E. Pyridylazo dye

F. Ferene

G. Nitroso-PSAP

Z. Other

SODIUM AND POTASSIUM

A. Ion selective electrode (diluted)

B. Ion selective electrode (undiluted)

C. Flame emission photometry

D. Ion selective electrode (flame mode)

Z. Other

CHLORIDE

A. Ion selective electrode (diluted)

B. Ion selective electrode (undiluted)

C. Coulometric-amperometric titration

D. Mercuric/ferric thiocyanate

E. Mercuric nitrate, diphenylcarbazone

F. Mercuric TPTZ

G. Ferric nitrite/methanesulfonic acid

H. Ferric perchlorate/methanesulfonic acid

Z . Other

ALBUMIN

A. Bromcresol green dye binding

B. Bromcresol purple dye binding

C. Nephelometric

Z. Other

TOTAL PROTEIN

A. Biuret (alkaline cupric sulfate)

B. Refractometry

Z. Other

CHOLESTEROL

A. Enzymatic (cholesterol esterase/oxidase/peroxidase)

B. Liebermann-Burchard reaction

Z. Other

LDL-CHOLESTEROL

A. Friedewald formula [LDL=Chol-HDL-(Trig5)]

E. DeLong formula [LDL=Chol-HDL-(Trig6)]

B. Immunoseparation ("Direct LDL")

C. Electrophoresis

D. Ultracentrifugation

Z. Other

HDL-CHOLESTEROL

ENZYMATIC MEASUREMENT WITH:

A. Heparin/Manganese precipitation

B. Dextran sulfate precipitation

J. Dextran sulfate w/ magnetic separation

C. Phosphotungstic acid + magnesium

H. Phosphotungstic acid

M. Polyethylene glycol

Z. Other

LIEBERMANN-BURCHARD REACTION WITH:

D. Heparin/Manganese precipitation

E. Dextran sulfate precipitation

K. Dextran sulfate w/ magnetic separation

F. Phosphotungstic acid + magnesium

G. Phosphotungstic acid

N. Polyethylene glycol

W. Other

TRIGLYCERIDES

WITHOUT CORRECTION FOR FREE GLYCEROL:

A. Enzymatic (with lactate dehydrogenase)

B. Enzymatic (with peroxidase)

C. Enzymatic (glycerol phosphate dehydrogenase/diaphorase)

D. Enzymatic (glycerol dehydrogenase/ diaphorase)

J. Enzymatic (glycerol dehydrogenase)

K. Enzymatic (glycerol phosphate oxidase)

W. Other

WITH CORRECTION FOR FREE GLYCEROL:

E. Enzymatic (with lactate dehydrogenase)

F. Enzymatic (with peroxidase)

G. Enzymatic (glycerol phosphate dehydrogenase/diaphorase)

H. Enzymatic (glycerol dehydrogenase/ diaphorase)

M. Enzymatic (glycerol dehydrogenase)

N. Enzymatic (glycerol phosphate oxidase)

Z. Other

ALANINE AMINOTRANSFERASE

A. Kinetic WITHOUT PYRIDOXAL PHOSPHATE - (lactate dehydrogenase/NADH)

B. Kinetic WITH PYRIDOXAL PHOSPHATE -(lactate dehydrogenase/NADH)

C. Colorimetric (dinitrophenylhydrazine)

Z. Other

ASPARTATE AMINOTRANSFERASE

A. Kinetic WITHOUT PYRIDOXAL PHOSPHATE - (malate dehydrogenase/NADH)

B. Kinetic WITH PYRIDOXAL PHOSPHATE -(malate dehydrogenase/NADH)

C. Kinetic (leuco dye)

Z. Other

alpha-AMYLASE

A. Chromogenic (p-nitrophenyl-substrate)
B. Chromogenic ("blocked" p-nitrophenyl-substrate)
C. Enzymatic/saccharogenic (maltose phosphorylase)

D. Enzymatic/saccharogenic (hexokinase)

E. Turbidimetric

F. Chromogenic (drimarene red-amylopectin)

Z. Other

ALKALINE PHOSPHATASE

A. 4-Nitrophenyl phosphate (pNPP)/AMP

(2-Amino-2-methyl-1-propanol) buffer

B. 4-Nitrophenyl phosphate (pNPP)/

2-amino-2-methyl-1,3-propanediol buffer

C. 4-Nitrophenyl phosphate (pNPP)/DEA (diethanolamine) buffer

D. 4-Nitrophenyl phosphate (pNPP)/

carbonate buffer

E. 4-Nitrophenyl phosphate (pNPP)/

N-Acetylglucamine buffer

F. Naphthyl monophosphate

Z. Other

gamma-GLUTAMYLTRANSFERASE

A. L--glutamyl-3-carboxy-4-nitroanalide/ glycylglycine

B. L--glutamyl-4-nitroanalide/ glycylglycine

Z. Other

CREATINE KINASE

A. Creatine phosphate/NADP

B. Creatine phosphate/NAD

C. Creatine phosphate/glycerol kinase/ peroxidase

Z. Other

CREATINE KINASE ISOENZYME MB

A. Direct immunochemical measurement of CK-MB protein (results in ng/mL)

P. Electrophoresis (results in %)

CATALYTIC ACTIVITY MEASUREMENT (U/L);

SEPARATION ACHIEVED BY:

B. Immunochemical inhibition

C. Immunochemical precipitation

D. Electrophoresis (results in U/L)

E. Column chromotography

Z. Other

LACTATE DEHYDROGENASE

A. Lactate to pyruvate

B. Pyruvate to lactate

LACTATE DEHYDROGENASE ISOENZYME 1

P. Electrophoresis (results in %)

LACTATE TO PYRUVATE REACTION (U/L);

SEPARATION ACHIEVED BY:

A. Immunochemical inhibition

B. Immunochemical precipitation

E. Electrophoresis (results in U/L)

F. Column chromotography

G. Chemical inhibition

W. Other

PYRUVATE TO LACTATE REACTION (U/L);

SEPARATION ACHIEVED BY:

H. Immunochemical inhibition

J. Immunochemical precipitation

K. Electrophoresis (results in U/L)

L. Column chromotography

M. Chemical inhibition

Z. Other