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Advanced Light Microscopy and Image Analysis Core

Time-line of imaging technology: phase contrast, differential interference, microinjected fluorescence anti-tubulin antibody, GFP tagged Histone, fixed preparation labeling three different proteins using antibodies/dye (DNA blue, actin red, microtubules green).
Timeline of imaging technology

Links and Equipment

Links

Imaging

What do you need to image? Click link(s) below to see how we can help.

| Tissue | Cells | Whole Mounts | Other |

Tissue (100-1000X)

Most fluorescently labeled tissues slices (100µm uncleared and up to 500µm cleared) are best imaged with a confocal microscope. The confocal microscope removes most of the "out-of-focus" blur associated with imaging fluorescent specimens that are thicker than the depth of field of the objective lens.

Multi-channel:
* up to 3 biomolecules/fluorophore can be collected simultaneously examples in gallery + reflection microscopy

*Z series (can be combined with multi biomolecules or fluorophores)

Cells (100-1000X)

Live (RT ->500C)
Transmitted light:

Fluorescence: Metal halide illumination

Fixed (fluorescent or histological stains)
Multi-channel:

True color:

Whole mounts

Confocal: object(s) <500uM

Dissecting scope: object(s) 300µM to mm's

Other

Opaque objects: small parts, tobacco, etc.

Phase objects: Crystals