Advanced Light Microscopy and Image Analysis Core
Timeline of imaging technology
Links and Equipment
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- ALM Home
- Imaging Needs?
- Image Analysis
- Image Gallery
- Equipment:
Related Links
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- Affiliation:
Smart Lighting Center
Imaging
What do you need to image? Click link(s) below to see how we can help.
Tissue (100-1000X)
Most fluorescently labeled tissues slices (100µm uncleared and up to 500µm cleared) are best imaged with a confocal microscope. The confocal microscope removes most of the "out-of-focus" blur associated with imaging fluorescent specimens that are thicker than the depth of field of the objective lens.
Multi-channel:
* up to 3 biomolecules/fluorophore can be collected simultaneously examples in gallery + reflection microscopy
*Z series (can be combined with multi biomolecules or fluorophores)
Cells (100-1000X)
Live (RT ->500C)
Transmitted light:
- *Phase contrast
- *Differential interference contrast (DIC)
- * Time-lapse
Fluorescence: Metal halide illumination
- *FRAP - example in gallery
- *Time lapse of one or more biomolecules or fluorophore - example in gallery
- *Z series (can be combined w/multi biomolecules or fluorophore) - example in gallery
Fixed (fluorescent or histological stains)
Multi-channel:
- * up to 5 biomolecules/fluorophore - examples in gallery
- *Z series (can be combined with multi biomolecules or fluorophore
True color:
Whole mounts
Confocal: object(s) <500uM
- * up to 3 biomolecules/fluorophores simultaneously + reflection microscopy
- * Z series (100µm uncleared and up to 500µm cleared)
- * magnification (40 -1000X) - example in gallery
Dissecting scope: object(s) 300µM to mm's
- * up to 3 biomolecules/fluorophores + transmitted light mode - example in gallery
- * magnification (10 -600X?) - examples in gallery
- * Extensive measurements tools
Other
Opaque objects: small parts, tobacco, etc.
- * magnification (10 -1000X?) - examples in gallery
- * Extensive measurements tools
Phase objects: Crystals