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Advanced Light Microscopy and Image Analysis Core

Time-line of imaging technology: phase contrast, differential interference, microinjected fluorescence anti-tubulin antibody, GFP tagged Histone, fixed preparation labeling three different proteins using antibodies/dye (DNA blue, actin red, microtubules green).
Timeline of imaging technology

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Neurons

Multi-photon excitation in live tissue (~325µM thick) Z series volume of rat spinal motoneurons innervating soleus muscle (red) and gastrocnemius muscle (green) retrogradely labeled by intramuscular injection with cholera toxin subunit B conjugated to Alexa 594 and FITC, respectively.

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