Biochemistry Shared Instrumentation
The Biochemistry Core contains a selection of shared instrumentation available to researchers at the Wadsworth Center as well as scientists from neighboring institutes. The purpose of the Core is to provide easy access to highly technical instrumentation and highly specialized methods not other wise readily accessible.
The Core was designed to study biological molecules by means of purification, analysis and the study of their physical properties. The methods employed for purification and analysis include liquid chromatography and isoelectric focussing. Physical measurements can be achieved by a variety of spectrophotometric techniques (circular dichroism, ultra-violet, visible, and fluorescence spectroscopy), dynamic light scattering, and analytical ultracentrifugation.
A Jasco circular dichroism spectrometer is one of many insturments in the Biochemistry Core Facility which investigators use to perform biophysical analyses of proteins and other molecules.
Staff provide initial training in concepts and use of the instruments, primarily through one to one tutorial instruction, with the overall objective that researchers will be able to perform their own experiments and run the instrumentation independently. A course on theoretical and specific instrumentation is periodically presented. The Core's mission is to continually seek out new methods and technologies which will support public health studies and make them available to the scientific community.
- Protein purification equipment (HPLCs, FPLC)
- Isoelectric Focusing
- Analytical Ultracentrifuge
- Fluorescence Spectroscopy
- UV/Vis spectroscopy
- Circular Dichroism Spectroscopy
- Dynamic Light Scattering
For further information contact: David LeMaster at lemaster@wadsworth.org - Biggs Laboratory, Empire State Plaza