Main Body
Biochemistry Shared
Biochemistry Shared
Biochemistry Shared Instrumentation
Biochemistry Shared
Instrumentation Facility
Biochemistry Shared
Instrumentation Facility
Main Menu
Instrumentation:
- ACQUITY Ultra
Performance LC® (UPLC®) - Size Exclusion
Chromatography Multi-Angle
Laser Light Scatter
(SECMALLS) - Analytical
Ultracentrifugation - Capillary Electrophoresis
- Preparative Isoelectric
Focusing - UV/VIS Spectroscopy and
Fluorescence Spectroscopy - Dynamic Light Scattering
- Circular Dichroism
Spectroscopy - Other Instrumentation
Preparative Isoelectric Focusing
Preparative Isoelectric Focusing: The Rainin protein fractionator (minipHor) is a fast method of purifying proteins in free solution (typically containing ampholytes or a mixture of buffers). It provides resolution based on different physical properties than column chromatography. It is possible to resolve proteins that differ by only a fraction of a pH unit. Large amounts of protein (up to 250 mg) may be handled in a single run. Typically the first experiment uses ampholines in a wide pH range. Subsequent purification improves by narrowing the pH range and selecting the ideal focusing chemicals.