Flow Cytometry Service Request Form
FLOW CYTOMETRY REQUEST FORM
Flow Cytometry Unit of Immunology Core at
DAI 5085 and BIGGS C505
Core Director: Dr. David A. Lawrence BIGGS C539
(402-5684)
Assistants: at BIGGS -- Joan Pedersen-Lane/Renjie
Song/Steve Rich
at DAI -- Kenneth Class
(Telephone: BIGGS C505 474-3697; DAI 5085 486-4915)
INFORMATION ABOUT FLOW CYTOMETERS
Becton Dickinson FACSVantage:
(DAI)
Laser excitation wavelengths: two lasers generating three
laser lines
Lines at 350 nm, 488 nm, 633 nm.
Eight-parameter detection: Forward scatter, Side scatter
and five fluorescent dyes.
A MacroSORT option is available for large particle (up to
150um)
analysis or sorting. An Automatic Cell Deposition Unit
(ACDU) That sorts a predefined number of cells into
individual wells of a 96 or 24 well microtiter plate is
also available. Location: DAI /5085
Becton Dickinson FACSCalibur:
(DAI)
Laser excitation wavelength: Argon 488nm and red diode 635
nm
Emission wavelength detection: Forward scatter (size), side
scatter (granularity), Four high performance, high dynamic
range photomultipliers with band pass filters: 530nm
(FITC), 585 nm (PE/PI), 661nm (APC), and 650 nm (PerCP)
with FL3 or >670nm with FL4.
FACSLoader provides automated or manual introduction of
prepared samples, featuring removable 40-tube carousels,
with on-board mixing.
Becton Dickinson FACScan: (DAI
& BIGGS)
Laser excitation wavelength: Argon 488nm
Emission wavelength detection: up to 5 parameters; forward
scatter (size), side scatter (granularity), FL1:
515-545nm(green), FL 2: 564-606nm(yellow/orange), FL3
>650nm(red).
NAME:
PRINCIPAL INVESTIGATOR:
LAB ROOM # ____________ PHONE # __________________________
FUNDING INFORMATION: REQUIRED BEFORE
USAGE
HRI
ACCOUNT#__________________________STATE
ACCOUNT#__________________
Please contact core
personnel about the charge fee for flow
usage.
PI will be billed
quarterly.
Sample Preparation Requirements:
SINGLE PARTICLES in a
NON-PROTEIN BUFFER
Concentration of
0.5-2.0 x 106 particles/ mL.
Volume at 0.5-1
mL/sample/tube.
For new protocols: All appropriate controls necessary for
parameter setups must be included as determined by Core
personnel.
SAMPLE INFORMATION: please be as detailed as possible, attach additional pages as necessary.
FLUORSCENT PROBE/DYE CHARACTERISTICS (spectra, Ex nm, Em
nm)
_______________________________________________
DESCRIPTION OF SAMPLE: (cells including their source,
DNA, and relative size)
_______________________________________________
DESCRIPTION OF SAMPLE PROTOCOL, GOALS AND
REFERENCE:
_________________________________________
_________________________________________
_________________________________________
_________________________________________
_________________________________________
APPROVED BY (ALL SIGNATURES ARE REQUIRED BEFORE
USAGE)
PI: _________________________________________ DATE:
_________
LAB CHIEF: _________________________________DATE:
_________
CORE DIRECTOR: __________________________ DATE: _________
Print & Send completed form to:
Flow Unit Assistants:
Joan Pedersen-Lane/Renjie Song/Steve Rich, (DAI) Kenneth
Class
BIGGS C505 474-3697,
DAI 5085 486- 4915
Or to Core Director,
Dr. David Lawrence BIGGS, C539. Phone 402-5684; Fax
474-1412