ImmunoElectron Microscopy
Immunolabeled THP-1 cells showing Glutathione distribution within the nucleus (N), mitochondria (M) and cytoplasm. Ault J., Lawrence, D.A. Exp Cell Res. 2003 Apr 15; 285(1): 9-14.
EM Immuno-localization is a service that can be performed with antibodies, which show precision staining at the light microscopic level, to determine if any cellular organelles or structures are associated with the antigen. Usually this is done on sections after cells are fixed or embedded in resin. This avoids the need to perforate membranes to allow antibodies and gold particles into the cell, which may allow soluble antigens to leak out of the cell or shift to other cellular compartments or intracellular spaces.
Sample requirements:
The client must provide the primary antibodies that previously have been shown to react with the specific antigen and the cells of interest. The core service will provide fixation of cells, labeling with the primary antibody (provided by client) and secondary gold conjugated antibody. A PDF file of the pictures will be provided to the client.
Contact personnel: Jeffrey G. Ault PhD. BIGGS/C138 486-9113