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LED’s impact in Transmitted light illumination

Current technology Vs. LEDs of future

Current:

  • Color temperature varies with intensity:
  • Cell damage due to phototoxicity

LEDs of future:

  • Constant color temperature (over wide output range)
  • Less damage to cells due spectral matching

Filtered and shuttered     versus    No filters or shutter:

Imaging cells delays exit from mitosis (Left: Filtered and shuttered versus Right: No filters or shutter)

Imaging cells delays exit from mitosis

Click here for Windows Media movie of cells progressing through mitosis -- Size: 3.49 MB



True-color images of a H&E stained histology section

Illuination -1.0 volt color balanced +1.0 volt;
Tungsten illumination
LED illumination