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Diagnostic Immunology/HIV Proficiency Testing Program

Grading Policy

Laboratories are evaluated on the basis of their responses for each analyte and on overall performance for all the analytes tested in the permit category. Appropriate responses are determined by participant consensus requiring 80% agreement in each test.

Grading Criteria:

  • Results for analytes your laboratory tests which are left blank will be considered incorrect and be given a score of 0%. If, for some reason, your laboratory is unable to process the samples for an analyte please notify us prior to submission of test results.
  • When both qualitative and quantitative results are reported ten points are deducted for each incorrect result. When only qualitative OR quantitative results are reported twenty points are deducted for each incorrect result.
  • For Diagnostic Services, failure to attain an overall testing score of at least 80% is unsatisfactory performance.
  • For Donor Services, failure to attain an overall testing score of 100% is unsatisfactory performance.
  • For HIV, failure to attain an overall testing score of 100% is unsatisfactory performance.
  • Laboratories failing two out of three consecutive proficiency test events for an analyte or for the permit category will fail the proficiency testing program for the analyte or for the permit category and may be required to cease patient testing for that analyte/category.

Determination of Acceptable Responses


Analyte or Test Criteria
Alpha-1-Antitrypsin Mean value ± 3 S.D
Antinuclear Ab Mean value ± 2 dilutions or
Positive/ Negative
Antistreptolysin O Mean value ± 3 S.D. or
Mean value ± 2 dilutions or
Positive/ Negative
Complement C'3, C'4 Mean value ± 3 S.D
Cytomegalovirus Ab Mean value ± 3 S.D. or
Reactive/ Nonreactive
Hepatitis (HBsAg, HBcAb, HBeAg, and HCAb) Reactive/ Nonreactive
HIV 1 or 1/2 Ab, Ag Reactive/ Nonreactive
HTLV 1 or 1/2 Ab (EIA or Western Blot) Reactive/ Nonreactive
Immunoglobulin A, E, M Mean value ± 3 S.D
Immunoglobulin G Mean value ± 25% Mean value
Infectious Mononucleosis Mean value ± 2 dilutions or
Positive/ Negative
LYME Disease Ab, WB IgG, WB IgM Reactive/ nonreactive
Rheumatoid Factor Mean value ± 3 S.D. or
Mean value ± 2 dilutions or
Positive/ Negative
Rubella Ab, Rubella IgM Mean value ± 3 S.D. or
Mean value ± 2 dilutions or
Positive/ Negative or
Immune/ Nonimmune
Syphilis - Reagin Ab Mean value ± 1 dilution or
Positive/ Negative
Syphilis - Treponemal Ab Reactive/ Nonreactive