Postdoctoral Positions at Wadsworth Center

Eukaryotic gene regulation

Yeast Molecular Biology and Biochemistry:

NIH/NSF - funded projects: The goals are to (1) determine the mechanism of action of the Ess1 proly-isomerase, which binds to RNA pol II and regulates transcription (2) employ a structure-function approach to develop Ess1 as a potential antifungal drug target. The projects will utilize biochemical analysis, microarrays, chromatin Ip, in vitro transcription and yeast genetics including SGA analysis. Experience in yeast and transcription preferred. Biochemical and genetic experience required; a personal interview is mandatory.

Selected Publications:

Wu, X., Wilcox, C. B., Devasahayam, G., Hackett, R.L., Arevalo-Rodriguez, M., Cardenas, M., Heitman, J., and Hanes, S. D. (2000). The Ess1 prolyl-isomerase is linked to chromatin remodeling complexes and the general transcription machinery. EMBO J. 19:3727-3738. (selected for "Editor's Choice" section, Science, 289:833)

Devasahayam, G., Chaturvedi, V. and Hanes, S. D., (2002). The Ess1 prolyl-isomerase is required for growth and morphogenetic switching in Candida albicans. Genetics 160:37-48.

Wu, X., Rossettini, A. and Hanes, S. D. (2003). The ESS1 prolyl isomerase and its suppressor BYE1 interact with RNA pol II to inhibit transcription elongation in Saccharomyces cerevisiae. Genetics 165:1687-1702.

Wilcox, C. B., Rossettini, A. and Hanes, S. D. (2004). Genetic interactions with CTD kinases and the CTD of RNA pol II suggest a role for ESS1 in transcription initiation and elongation in Saccharomyces cerevisiae. Genetics 167:93-105.

Gemmill, T., Wu, X., and Hanes, S.D. (2005). Vanishingly low levels of Ess1 prolyl-isomerase activity are sufficient for growth in Saccharomyces cerevisiae. J. Biol. Chem. 280:15510-15517.

Ren, P., Chaturvedi, V. and Hanes, S. D. (2005). The Ess1 prolyl isomerase is dispensable for growth but required for virulence in Cryptococcus neoformans. Microbiology 151:1593-1605.

Li, H-M, Li, Z., Devasahayam, G., Gemmill, T., Chaturvedi, V., Hanes, S. D., and Van Roey, P. (2005). Structure of the Candida albicans Ess1 prolyl isomerase reveals a well-ordered linker region that restricts domain mobility. Biochemistry 44:6180-6189.