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Chromatin structure and transcriptional regulation in yeast Description: A grant-funded position is available to work on either of two projects currently underway in the lab. The first is aimed at understanding how transcription factors, including both activators and general transcription factors, gain access to nucleosomal sites in vivo. We approach this question using a combination of yeast genetics, chromatin structural analysis, and chromatin immunoprecipitation, among other techniques. Our work to date has been done in yeast, but we are also interested in developing a combination of in vitro approaches with chromatin isolation from yeast to delineate the factors needed to allow transcription factor binding to nucleosomal sites. Relevant publications include: Stafford, G.A. and R.H. Morse. 1997. Chromatin remodeling by transcriptional activation domains in a yeast episome. J. Biol. Chem. 272, 11526-11534. Ryan, M.P., R. Jones, and R.H. Morse. 1998. SWI/SNF complex participation in transcriptional activation at a step subsequent to activator binding. Mol. Cell. Biol. 18: 1774-1782. Yu, L. and R.H. Morse. 1999. Chromatin opening and transactivator potentiation by RAP1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 5279-5288. Ryan, M.P., G.A. Stafford, L. Yu, and R.H. Morse. 2000. Artificially recruited TATA-binding protein fails to remodel chromatin and does not activate three promoters that require chromatin remodeling. Mol. Cell. Biol. 20: 5847-5857. Stafford, G.A. and R.H. Morse. 2001. GCN5 dependence of chromatin remodeling and transcriptional activation by the GAL4 and VP16 activation domains in budding yeast. Mol. Cell. Biol. 21: 4568-4578. Yu, L., N. Sabet, A. Chambers, and R.H. Morse. 2001. The N-terminal and C-terminal domains of RAP1 are dispensable for chromatin opening and GCN4-mediated HIS4 activation in budding yeast. J. Biol. Chem. 276: 33257-264. Our second main area of interest is in the regulatory role of the histone amino termini. We have discovered that several yeast genes are repressed by novel mechanisms involving the histone amino termini and are utilizing a combination of genetics and microarray analysis to dissect these mechanisms in detail. This is a new area for the lab and we currently have one manuscript under review on this project. The Wadsworth Center houses state-of-the-art core facilities in cell, molecular and structural biology and bioinformatics, including media preparation, oligonucleotide preparation and DNA sequencing facilities, and a microarray facility. The Wadsworth Center also sponsors an outstanding seminar program. In addition, two academic departments of the University at Albany School of Public Health are located here. As the capitol of New York State, Albany offers a diverse range of cultural activities; at the same time, it is small enough (about 100,000 in Albany proper) that cost of living and style of life are in many ways more congenial than often found in large metropolitan areas. Albany also offers easy access to numerous outdoor activities, such as skiing and hiking in the Adirondack, Catskill and Berkshire Mountains. Applicants must have a Ph.D. in the biomedical sciences. Experience in molecular biology and/or genetics is desired. Please send a current C.V. and the names of 3 references to: Randall H. Morse, Wadsworth Center, P.O. Box 22002, Albany NY 12201 or Randall.Morse@wadsworth.org.
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