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Postdoctoral Positions at Wadsworth Center

Transcription and chromatin in yeast

A position is available to investigate transcriptional regulation and control of chromatin structure by Rap1 and Abf1 in budding yeast. These two general regulator factors share an ability to create a local region of open chromatin which can facilitate binding of other activators to gene promoters and thus contribute to gene activation. However, these two GRFs also differ in some respects. Most dramatically, continued binding of Rap1 is required to facilitate ongoing transcriptional activation, whereas loss of Abf1 binding (using an abf1-1 ts mutant) does not result in decreased transcription. Thus, at least some promoters retain a "memory" of Abf1 binding. The successful candidate will use a combination of biochemical, genetic, and genomic approaches to gain new insight into the mechanism by which Rap1 and Abf1 contribute to transcriptional activation and perturb local chromatin structure, and into the basis of this "memory effect".

References

  • Yarragudi, A., T. Miyake, R. Li, and R.H. Morse. 2004. Comparison of ABF1 and RAP1 in chromatin opening and transactivator potentiation in the budding yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 24: 9152-9164.
  • Yarragudi, A., L.W. Parfrey, and R.H. Morse. 2007. Genome-wide analysis of transcriptional dependence and probable target sites for Abf1 and Rap1 in Saccharomyces cerevisiae. Nucleic Acids Res. 35: 193-202.