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Mycobacteriology Laboratory

Mycobacteriology Proficiency Testing Program

October 2005

Sample preparation (strains used):

SAMPLE #	GROWTH DETECTION / IDENTIFICATION		SOURCE
SP 0561	No acid-fast bacilli present
SP 05621	Acid-fast bacilli present	M. tuberculosis	(Patient sample IS 250288)
SP 0563	No acid-fast bacilli present
SP 05641	Acid-fast bacilli present	M. gordonae2 / contaminants3	(ATCC 14470)
SP 05651	Acid-fast bacilli present	M. bovis / contaminants3	(Patient sample IS 210720)

¹ These samples were selected for the identification score
² Sample not validated
³ S. sanguis, B. cereus

Sample preparation (strains used):

SCORES	[Growth Detection / Identification]
	SP 0561	SP 0562	SP 0563	SP 0564	SP 0565
M. bovis	-	20/10	-	-	20/20
M. bovis BCG	-	-	-	-	20/15
M. tuberculosis	-	20/20	-	-	20/10
M. tb complex	-	20/204	-	-	20/204
M. tb complex not M. tb	-	20/10	-	-	20/204
M. gordonae	-	-	-	20/20	-
M. marinum	-	-	-	-2	-
NTM	-	20/0	-	20/20	20/0
No Growth	20	0/20	20	-2	0/20
Other5

⁴ General, and General-S: 20/15
⁵ Score varies according to the information provided

Sample preparation (strains used):

SAMPLE #	IDENTIFICATION		SOURCE
Restricted, Restricted-S
IS 0571		M. tuberculosis	ATCC 25177
IS 0572		M. africanum	Patient sample (IS 210271)
General, General-S
IS 0573		M. tuberculosis (Mono-Resistant PZA)	Patient sample (IS 250288)
IS 0574		M. africanum	Patient sample (IS 210271)

Sample preparation (strains used):

SCORES	[Identification]
	IS 0571	IS 0572	IS 0573	IS 0574
M. bovis	-	-	10	10
M. bovis BCG	-	10	-	10
M. tuberculosis	20	10	20	10
M. tb complex	20	20	15	15
M. tb complex not M. tb	-	20	10	20
M. africanum	-	20	-	20
NTM	-	0	-	-
Contaminated	-	-	-	-
M. bovis and M. abscessus	-	-	5	-
No Growth	-	-	0	-