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Mycobacteriology Laboratory

Mycobacteriology Proficiency Testing Program

March 2006

Sample preparation (strains used):

SAMPLE #	GROWTH DETECTION / IDENTIFICATION		SOURCE
SP 06211	Acid-fast bacilli present	M. kansasii / contaminants2	(Patient sample SP 200480)
SP 06221	Acid-fast bacilli present	M. tuberculosis	(ATCC 25177)
SP 06231	Acid-fast bacilli present	M. tuberculosis / contaminants2	(ATCC 25177)
SP 0624	No acid-fast bacilli present	contaminants2
SP 06251	Acid-fast bacilli present	M. tuberculosis / contaminants2	(ATCC 25177)

¹ These samples were selected for the identification score
² S. sanguis, B. cereus
³ Not validated

Sample preparation (strains used):

SCORES	[Growth Detection / Identification]
	SP 0621	SP 0622	SP 0623	SP 0624	SP 06253
No Growth	-	0/20	0/20	20	20
M. tb complex	-	20/204	20/204	-	20
M. tuberculosis	-	20/20	20/20	-	20
M. tb and M. kansassii	-	20/15	-	-	20
M. kansasii	20/20	-	-	0	20
NTM	20/204	-	-	-	-

⁴ General, and General-S: 20/15

Sample preparation (strains used):

SAMPLE #	IDENTIFICATION		SOURCE
Restricted, Restricted-S
IS 06313		M. gordonae	(ATCC 14470)
IS 0632		M. bovis	Patient sample (IS 241223)
General, General-S
IS 0633		M. xenopi	Patient sample (IS 250288)
IS 0634		M. bovis	Patient sample (IS 241223)

Sample preparation (strains used):

SCORES	[Identification]
	IS 06313	IS 0632	IS 0633	IS 0634
No Growth	20	0	0	0
M. tb complex	-	20	-	15
M. complex not M. tb	-	20	-	20
M. tuberculosis	-	-	-	10
M. avium	20	-	-	-
M. bovis	-	20	-	20
M. scrofulaceum	-	-	0	-
M. xenopi	-	-	20	-
M. africanum	-	-	-	10
NTM	20	0	15	-
Contaminated	20	0	-	-
Nocardia sp.	20	-	-	-

³ Not validated