Sfold Frequently Asked Questions (and Answers)

1. How is the algorithm for Sfold different from other established algorithms?

2. What are the unique capabilities of the algorithm?

3. What is the rationale of Sfold target-accessibility prediction for the design of RNA-targeting nucleic acids?

4. Is there experimental evidence that the potency of siRNAs is influenced by target accessibility and secondary structure?

5. How about published empirical rules for siRNA duplex thermodynamics and features?

6. What is Sfold methodology for siRNA design? How is it different from other on-line design tools?
7. Have the nucleic acid design methods been experimentally validated?

8. What else is planned for Sfold?

9. How can users contribute to the improvement of the software, for the benefit of the scientific community?


Updated by Ye Ding, April 15, 2004.

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