Main Body

Investigators and Program Directors

Select Publications       

• D.A. Maslov and R.K. Agrawal (2012). Mitochondrial translation in trypanosomatids, In RNA Metabolism in Trypanosomes, Bindereif, A. ed. (Springer-Verlag, Heidelberg), in press.
• T. Yokoyama T.R. Shaikh, N. Iwakura, H. Kaji, A. Kaji and R.K. Agrawal (2012). Structural insights into initial and intermediate steps of the ribosome recycling process. EMBO J. 31:1836–1846. 
• A. Yassin, M.E. Haque, P.P. Datta, K. Elmore, N.K. Banavali, L.L. Spremulli and R.K. Agrawal (2011). Insertion domain within mammalian mitochondrial translation initiation factor 2 serves the role of eubacterial initiation factor 1. Proc. Natl. Acad. Sci. USA 108:3918-3923.
• R.K. Agrawal, M.R. Sharma, A. Yassin, I. Lahiri and L.L. Spremulli (2011). Structure and function of organellar ribosomes as revealed by cryo-EM. In Ribosomes: Structure, Function, and Dynamics, Rodnina, M., Wintermeyer, W., & Green, R. eds. (SpringerWien, New York), pp. 83-96.
• M.R. Sharma, A. Dönhöfer, C. Barat, V. Marquez, P.P. Datta, P. Fucini, D.N. Wilson and R.K. Agrawal (2010). PSRP1 is not a ribosomal protein, but a ribosome binding factor that is recycled by RRF and EF-G. J. Biol. Chem. 285:4006-4014.
• M.R. Sharma, T.M. Booth, L. Simpson, D.A. Maslov and R.K. Agrawal (2009). Structure of a mitochondrial ribosome with minimal RNA. Proc. Natl. Acad. Sci. USA 106:9637-9642. [Highlighted in “In this issue” section]
• R. Gaur, D. Grasso, P.P. Datta, P.D.V. Krishna, G. Das, A. Coursey, R.K. Agrawal, L. Spremulli and U. Varshney (2008). A single mammalian mitochondrial translation initiation factor functionally replaces two bacterial factors. Mol. Cell 29:180-190.
  [Highlighted on the cover of the Cold Spring Harbor Translational  Control meeting abstract book, 2008]
• M.R. Sharma, D.N. Wilson, P.P. Datta, C. Barat, F. Schluenzen, P. Fucini and R.K. Agrawal (2007). Cryo-EM study of the Spinach chloroplast ribosome reveals the structural and functional roles of plastid-specific ribosomal proteins. Proc. Natl. Acad. Sci. USA 104:19315-19320.
• P.P. Datta, D.N. Wilson, M. Kawazoe, N.K. Swami, T. Kaminishi, M.R. Sharma, T.M. Booth, C. Takemoto, P.Fucini, S. Yokoyama and R.K. Agrawal (2007). Structural aspects of RbfA action during small ribosomal subunit assembly. Mol. Cell 28:434-445.
• C. Barat, P.P. Datta, V.S.Raj, M.R. Sharma, H. Kaji, A. Kaji and R.K. Agrawal (2007). Progression of the Ribosome Recycling Factor Through the Ribosome Dissociates the Two Ribosomal Subunits. Mol. Cell 27:250-261.
• J.A. Mears, M.R. Sharma, R.R. Gutell, A.S. McCook, P.E. Richardson, T.R. Caulfield, R.K. Agrawal and S.C. Harvey (2006). A Structural Model for the Large Subunit of the Mammalian Mitochondrial Ribosome. J. Mol. Biol. 358:193-212.
• P.P. Datta, M.R. Sharma, L. Qi,  J. Frank and R.K. Agrawal (2005). Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal protein L7/L12 during translocation as revealed by cryo-EM. Mol. Cell 20:723-731.
• M.R. Sharma, C. Barat, D.N. Wilson, T.M. Booth, M.Kawazoe, C. Hori-Takemoto, M. Shirouzu, S. Yokoyama, P. Fucini and R.K. Agrawal (2005). Interaction of Era with the 30S ribosomal subunit: Implications for 30S subunit assembly. Mol. Cell 18:319-329.
• R.K. Agrawal, M.R. Sharma, M.C. Kiel, G. Hirokawa, T.M. Booth, C.M. Spahn, R.A. Grassucci, A. Kaji and J. Frank (2004). Visualization of ribosome recycling factor on the Escherichia coli 70S ribosome: Functional implications. Proc. Natl. Acad. Sci. USA 101:8900-8905.
• M.R. Sharma, E.C. Koc, P.P. Datta, T.M. Booth, L.L. Spremulli and R.K. Agrawal (2003). Structure of the mammalian mitochondrial ribosomes reveals an expanded role for its component proteins. Cell 115:97-108.
  [Commentaries in “The Scientist” and “Creation-Evolution Headlines”]
• J. Sengupta, R.K. Agrawal and J. Frank (2001). Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA. Proc. Natl. Acad. Sci. USA 98:11991-11996 [Cover-page citation].
• J. Frank and R.K. Agrawal (2001). Ratchet-like movements between the two ribosomal subunits: their implications in the elongation factor recognition and tRNA translocation. Cold Spring Harbor Symp. Quant. Biol. LXVI:67-75.
• I.S. Gabashvili, R.K. Agrawal, C.M.T. Spahn, R.A.Grassucci, D.I. Svergun, J. Frank and P. Penczek (2000). Solution structure of the E. coli ribosome at 11.5 Å resolution. Cell 100:51-63.
• J. Frank and R.K. Agrawal (2000). A ratchet-like inter-subunit reorganization of the ribosome during translocation. Nature 406:318-322.
  
• R.K. Agrawal, C.M.T. Spahn, P. Penczek, R.A.Grassucci, K.H. Nierhaus and J. Frank (2000). Visualization of tRNA movements on the E. coli 70S ribosome during the elongation cycle. J. Cell Biol. 150:447-459
• R.K. Agrawal, A.B. Heagle, P. Penczek, R.A. Grassucci, and J. Frank (1999). EF-G-dependent GTP hydrolysis induces translocation accompanied by large conformational changes in the 70S ribosome. Nature Struct. Biol. 6:643-647
• R.K. Agrawal, P. Penczek, R.A. Grassucci and J. Frank (1998). Visualization of elongation factor G on the E. coli ribosome: The mechanism of translocation. Proc. Natl. Acad. Sci. USA 95:6134-6138.
  [Cover-page illustration; a commentary in a subsequent issue]
• R.K. Agrawal, P. Penczek, R. Grassucci, Y. Li, A. Leith, K.H. Nierhaus and J. Frank (1996). Direct visualization of A‑, P‑ and E‑site tRNAs in the E. coli ribosome. Science 271:1000-1002.
  [Highlighted in “This Week in Science” section; commentary in Nature Struct. Biol.]
• R. K. Agrawal and D. P. Burma (1996). Sites of ribosomal RNA involved in the subunit association of tight and loose couple ribosomes. J. Biol. Chem. 271:21285-21291.
• J. Frank, J. Zhu, P. Penczek, Y. Li, S. Srivastava, A. Verschoor, M. Radermacher, R. Grassucci, K. R. Lata and R. K. Agrawal (1995). A model of protein synthesis based on cryo‑electron microscopy of the E. coli ribosome. Nature 376:441‑444.

Contact Information

Phone: (518) 486-5797
Fax: (518) 474-5049
E-mail:agrawal@wadsworth.org