Investigators and Program Directors

Select Publications

• M.R. Sharma, D.N. Wilson, P.P. Datta, C. Barat, F. Schluenzen, P. Fucini, and R.K. Agrawal (2007). Cryo-EM study of the Spinach chloroplast ribosome reveals the structural and functional roles of plastid-specific ribosomal proteins. Proc. Natl. Acad. Sci. USA 104:19315-19320.
• P.P. Datta, D.N. Wilson, M. Kawazoe, N.K. Swami, T. Kaminishi, M.R. Sharma, T.M. Booth, C. Takemoto, P.Fucini, S. Yokoyama, & R.K. Agrawal (2007). Structural aspects of RbfA action during small ribosomal subunit assembly. Mol. Cell 28:434-445.
• C. Barat, P.P. Datta, V.S.Raj, M.R. Sharma, H. Kaji, A. Kaji, & R.K. Agrawal (2007). Progression of the Ribosome Recycling Factor Through the Ribosome Dissociates the Two Ribosomal Subunits. Mol. Cell 27:250-261.
• J.A. Mears, M.R. Sharma, R.R. Gutell, A.S. McCook, P.E. Richardson, T.R. Caulfield, R.K. Agrawal, & S.C. Harvey (2006). A Structural Model for the Large Subunit of the Mammalian Mitochondrial Ribosome. J. Mol. Biol. 358:193-212.
• P.P. Datta, M.R. Sharma, L. Qi, J. Frank, & R.K. Agrawal (2005). Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal protein L7/L12 during translocation as revealed by cryo-EM. Mol. Cell 20:723-731.
• M.R. Sharma, C. Barat, D.N. Wilson, T.M. Booth, M.Kawazoe, C. Hori-Takemoto, M. Shirouzu, S. Yokoyama, P. Fucini, & R.K. Agrawal (2005). Interaction of Era with the 30S ribosomal subunit: Implications for 30S subunit assembly. Mol. Cell 18:319-329.
• R.K. Agrawal, M.R. Sharma, M.C. Kiel, G. Hirokawa, T.M. Booth, A. Silasagaram, R.A. Grassucci, A. Kaji, & J. Frank (2004). Visualization of ribosome recycling factor on the Escherichia coli 70S ribosome: Functional implications. Proc. Natl. Acad. Sci. USA. 101:8900-8905.
• M.R. Sharma, E.C. Koc, P.P. Datta, T.M. Booth, L.L. Spremulli, and R.K. Agrawal 2003. Structure of the mammalian mitochondrial ribosomes reveals an expanded role for its component proteins. Cell 115:97-108 [commentaries in "The Scientist" and "Creation-Evolution Headlines"].
• J. Sengupta, R.K. Agrawal, and J. Frank 2001. Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA. Proc. Natl. Acad. Sci. U.S.A. 98:11991-11996 [cover-page citation].
• Frank and R.K. Agrawal 2001. Ratchet-like movements between the two ribosomal subunits: their implications in the elongation factor recognition and tRNA translocation. Cold Spring Harbor Symp. Quant. Biol. LXVI:67-75.
• I.S. Gabashvili, R.K. Agrawal, C.M.T. Spahn, R.A.Grassucci, D.I. Svergun, J. Frank, and P. Penczek 2000. Solution structure of the E. coli ribosome at 11.5 Å resolution. Cell 100:51-63.
• J. Frank and R.K. Agrawal 2000. A ratchet-like inter-subunit reorganization of the ribosome during translocation. Nature 406:318-322.
• R.K. Agrawal, C.M.T. Spahn, P. Penczek, R.A.Grassucci, K.H. Nierhaus, and J. Frank 2000. Visualization of tRNA movements on the E. coli 70S ribosome during the elongation cycle. J. Cell Biol. 150:447-459
• R.K. Agrawal, A.B. Heagle, P. Penczek, R.A. Grassucci, and J. Frank 1999. EF-G-dependent GTP hydrolysis induces translocation accompanied by large conformational changes in the 70S ribosome. Nature Struct. Biol. 6:643-647.
• R.K. Agrawal, P. Penczek, R.A. Grassucci, and J. Frank 1998. Visualization of elongation factor G on the E. coli ribosome: The mechanism of translocation. Proc. Natl. Acad. Sci. U.S.A. 95:6134-6138 [cover-page illustration; commentary in a subsequent issue].
• R.K. Agrawal, P. Penczek, R. Grassucci, Y. Li, A. Leith, K.H. Nierhaus, and J. Frank 1996. Direct visualization of A., P. and E.site tRNAs in the E. coli ribosome. Science 271:1000-1002 [highlighted in "This Week in Science" section; commentary in Nature Struct. Biol.].
• R. K. Agrawal and D. P. Burma 1996. Sites of ribosomal RNA involved in the subunit association of tight and loose couple ribosomes. J. Biol. Chem. 271:21285-21291.
• J. Frank, J. Zhu, P. Penczek, Y. Li, S. Srivastava, A. Verschoor, M. Radermacher, R. Grassucci, K. R. Lata, and R. K. Agrawal 1995. A model of protein synthesis based on cryo.electron microscopy of the E. coli ribosome. Nature 376:441.444.

Contact Information

Phone: (518) 486-5797
Fax: (518) 474-7992
E-mail:agrawal@wadsworth.org