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Investigators and Program Directors

Pei-Yong Shi

Research Scientist, Wadsworth Center, Viral Diseases
Associate Professor, School of Public Health, Biomedical Sciences

Ph.D., Georgia State University (1995)
Postdoctoral training, Yale University

<< Research Interests

Recent publications

  • Zhang, B., Dong, H., Zhou, Y., and Shi P.Y. 2008. Genetic interactions among the West Nile virus methyltransferase, the RNA-dependent RNA polymerase, and the 5’ stem-loop of genomic RNA. J. Virol. (In press) .
  • Dong, H., Ren, S., Li, H., and Shi P.Y. 2008. Separate molecules of West Nile virus methyltransferase can independently catalyze the N7 and 2’-O methylations of viral RNA cap. Virology (In press) .
  • Chotkowski, H.L., Ciota, A.T., Jia, J., Puig-Basagoiti, F., Kramer, L.D., Shi, P.Y., and Glaser, R.L. 2008. West Nile virus infection of Drosophila melanogaster induces a protective RNAi response. Virology (In press) .
  • Jerzak, G.; Brown, I.; Shi, P.Y.; Kramer, L.D.; Ebel, G.D. 2008. Genetic diversity and purifying selection in West Nile virus are maintained during host switching. Virology (In press).
  • Dong, H., Ren, S., Zhang, B., Zhou, Y., Puig-Basagoiti, F., Li, H., and Shi P.Y. 2008. West Nile virus methyltransferase catalyzes two methylations of the viral RNA cap through a substrate repositioning mechanism. J. Virol. 82, 4295-4307.
  • Zhang, B., Dong, H., Stein, D.A., Iversen, P.L., and Shi P.Y. 2008. West Nile virus genome cyclization and RNA replication require two pairs of long-distance RNA interactions. Virology 273, 1-13.
  • Stein, D.A. and Shi, P.Y. 2008. Nucleic acid-based inhibition of flavivirus infections. Front Biosci. 13:1385-95.
  • Davis, W.G., Blackwell, J.L., Shi, P.Y., and Brinton, M.A. 2007. Interaction between the cellular protein eEF1A and the 3' terminal stem loop of the West Nile virus genomic RNA facilitates viral RNA minus strand synthesis. J. Virol. 81,10172-87.
  • Deas, T.S., Bennett, C.J. Jones, S.A., Tilgner, M., Ren, P., Behr, M.J., Stein, D.A., Iversen, P.L. Kramer, L.D., Bernard, K.A., and Shi, P.Y. 2007. In vitro resistance selection and in vivo efficacy of morpholino oligomers against West Nile virus. Antimicrob. Agents Chemother. 51, 2470-82.
  • Dong, H., Ray, D., Ren, S., Zhang, B., Puig-Basagoiti, F., Takagi, Y., Ho, C.K. Li, H., and Shi P.Y. 2007. Distinct RNA elements confer specificity to flavivirus RNA cap methylation events. J. Virol. 81, 4412-21.
  • Zhou, Y., Ray, D., Zhao, Y., Dong, H., Ren S., Li, Z., Guo, Y., Bernard, K.A., Shi, P.Y., and Li, H. 2007. Structure and function of flavivirus NS5 methyltransferase. J. Virol. 81, 3891-903.
  • Puig-Basagoiti, F., Tilgner, M., Bennett, C.J., Zhou, Y., Muñoz-Jordán, J.L., García-Sastre, A., Bernard, K.A., and Shi, P.Y. 2007. A mouse cell-adapted NS4B mutation attenuates West Nile virus virulence through suppression of viral RNA synthesis. Virology 361, 229-41.
  • Kramer, L.D., Li, J., and Shi, P.Y. 2007. West Nile virus. The Lancet Neurology 6, 171-181.
  • Ciota, A.T., Ngo, K.A. Lovelace, A.O., Payne, A.F., Zhou, Y., Shi, P.Y., and Kramer, L.D. 2007. Role of the mutant spectrum in adaptation and replication of West Nile virus. J. Gen. Virol. 88, 865-874.
  • Jerzak, G.V.S., Bernard, K.A., Kramer, L.D., Shi, P.Y., and Ebel, G.D. 2007. The West Nile virus mutant spectrum is host-dependant and a determinant of mouse mortality. Virology 360, 469-476.
  • Gardner, I.A., Wong, S.J., Ferraro, G.L., Balasuriya, U.B., Hullinger, P.J., Wilson, W.D., Shi, P.Y., MacLachlan, N.J. 2006. Incidence and effects of West Nile virus infection in vaccinated and unvaccinated horses in California. Vet. Research. 38,109-116.
  • Ray, D., Shah, A., Tilgner, M., Guo,Y., Zhao, Y., Dong, H., Deas, T.S., Zhou, Y., Li, H., and Shi, P.Y. 2006. West Nile Virus 5’-Cap Structure is Formed by Sequential Guanine N-7 and Ribose 2’-O Methylations by Nonstructural Protein 5. J. Virol. 80, 8362-8370.
  • Balasuriya, U.B.R., Shi, P.Y., Wong, S.J., Demarest, V.L., Gardner, I.A., Hullinger, P.J., Ferraro, G.L., Boone, J.D., De Cino, C.L., Glaser, A.L., Renshaw, R.W., Ledizet, M., Koski, R.A., and MacLachlan, N.J. 2006. Detection of Antibodies to West Nile Virus in Equine Sera using Microsphere Immunoassay. J. Vet. Diagn. Invest. 18, 392-395.
  • Puig-Basagoiti, F., Tilgner, M., Forshey, B.M., Philpott, S.M., Espina, N.G., Wentworth, D.E., Goebel, S.J., Masters,P.S., Falgout, B., Ren, P., Ferguson, D.M., and Shi, P.Y. 2006. A triaryl pyrazoline compound inhibits flavivirus RNA replication. Antimicrob. Agents Chemother. 50, 1320-1329.
  • Julander, J.G., Winger, Q.A., Rickords, L.F., Shi, P.Y., Tilgner, M., Binduga-Gajewska, I., Sidwell, R.W., Morrey, J.D. 2006. West Nile virus infection of the placenta. Virology. 347, 175-82.
  • Goodell, J. R., Puig-Basagoiti, F., Forshey, B.M. Shi, P.Y., and Ferguson, D.M. 2006. Identification of compounds with anti-West Nile virus activity. J. Med. Chem. 49, 2127-2137.
  • Ray, D. and Shi, P.Y. 2006. Recent Advances in Flavivirus Antiviral Drug Discovery and Vaccine Development. Recent Patents on Anti-Infective Drug Discovery. 1, 45-55
  • Zhang, C., Cai, Z., Kim, Y.-C., Kumar, R., Yuan, F., Shi, P.Y., Kao, C., and Luo, G. 2005. Stimulation of the hepatitis C virus NS3 helicase by NS3 protease domain and RNA-dependent RNA polymerase. J. Virol. 79, 8687-8697.
  • Deas, T.S., Binduga-Gajewska I., Tilgner M., Ren, P., Stein, D.A., Moulton, H.M., Iversen, P.L., Kauffman, E.B., Kramer, L.D., and Shi, P.Y. 2005. Inhibition of flavivirus infections by antisense oligomers specifically suppressing viral translation and RNA replication. J. Virol. 79, 4599-4609.
  • Liu, W.J., Wang, X.J., Shi, P.Y., Randall, R., and Khromykh, A.A. 2005. West Nile and Kunjin virus RNA replication inhibits interferon signaling by blocking STAT1 and STAT2 phosphorylation. J. Virol. 79, 1934-1942.
  • Puig-Basagoiti, F., Deas, T.S., Ren, R., Tilgner, M., Ferguson, D.M., and Shi, P.Y. 2005. Comparison of genetics-based high-throughput assays for West Nile virus drug discovery. Antimicrob. Agents Chemother. 49, 4980-4988.
  • Tilgner, M., Deas, T.S., and Shi, P.Y. 2005. The flavivirus-conserved penta-nucleotide in the 3' stem-loop of the West Nile virus genome requires a specific sequence and structure for RNA synthesis, but not for viral translation. Virology 331, 375-386.