Resource for the Visualization of Biological Complexity (RVBC)

F20: FEI Tecnai TF-20 200 keV field-emission gun electron microscope

Main features

• Field-emission gun
• 2k CCD camera
• Intensified TV-rate camera
• Automated data collection
• Cameras and automation software fully-embedded in user interface
• Rotation-free imaging with tilt axis parallel to film long axis from 1500x to 620kX

Performance:

Objective lens

Focal length -- 2.7 mm

Chromatic aberration -- 2.0 mm

Spherical aberration -- 2.0 mm

Resolution

TEM lattice -- 0.14 nm

TEM point -- 0.27 nm

Cameras

 

Gatan MSC 794 2k CCD

Gatan 622 intensified TV camera

Specimen holders

Cryotransfer

Gatan CT3500 with extended Dewar (2)

Gatan 626

Room temperature

Standard

Gatan 670 high-tilt tomography (+/- 71) with Faraday cage

Software

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FEI Low Dose Software (G2 software v. 2.1.3)

FEI Tomography and 3D imaging software (Xplore3D v. 2.0)

Automated low dose data collection (in-house development is ongoing)