Resource for the Visualization of Biological Complexity (RVBC)

HVEM: AEI EM-7 Mk II High-Voltage Electron Microscope

100-1200 keV accelerating voltage
High-sensitivity TV camera for low-dose tracking and focus
Wide variety of specimen holders, most designed and constructed in-house
Rotation-free imaging with specimen tilt axis parallel to film long axis from 6.3 kX to 63 kX
Lens and deflector currents, and beam current, automatically scale with accelerating voltage
Turret allows six filament changes without opening system
Noran EDX system with in-house constructed detector shielding

Objective lens: Focal length -- 8.5 mm; Spherical aberration -- 13.2 mm; Chromatic aberration -- 6.7 mm; Polepiece gap -- 20 mm; Resolution (point to point): -- 0.5 nm; Magnification: -- 63-630X; 630X-1MX; Camera length: -- 50-500 cm; 50-500 m

Specimen holders: High-tilt tomography; Tilt-rotation; Double-tilt using indexed 90-degree rotation; 360-degree-tilt capillary holder; Holder for 5 mm diameter grids; Side-entry differentially-pumped environmental chamber; Gatan 626 cryo-transfer with two-blade anticontaminator; Capability for design and construction of additional special holders

In-house constructed intensified CCD camera for very low dose search and focus at TV rate, with adequate quality for televiewing and for acquiring reference images for specimen mapping
In-house constructed high-speed film scanner for tomographic tilt series and stereopairs, with interactive real-time pre-alignment and histogram adjustment

A: Stage controller
B: Filament and beam current display
C: High-sensitivity TV camera
D: EDX detector
E: Image wobbler

F: Temperature controllers
G: Accelerator (above column)
H: Filament turret airlock
I: HT Generator