DENDRITE

Subject:

Dendrite

View:

Shaded surface representations seen from 20 different views of an electron tomographic 3D reconstruction. Successive views form stereo pairs.

SPIDER/WEB users: Threshold = -0.1

Specimen source:

Rat neostriatum.

Specimen preparation:

Selectively stained by injection of horseradish peroxidase, embedded in Spurr's resin and cut into 2-3 um thick sections.

Electron microscopy:

Tilt series for tomographic reconstruction was recorded over a 124 degree angular range, using a 2 degree angular interval, with a high voltage electron microscope.

Acceleration voltage = 1.0 MV. Em = AEI.

Image processing:

Alignment using gold markers and cross-correlation. Reconstruction technique: Modified back-projection. Direction of missing wedge: Horizontal in top left view on gallery.

Magnification:

1600X. Pixel size = 15 nm.

Reference:

Wilson, C.J., Mastronarde, D.N., McEwen, B.F., Frank, J. (1992) Neuroimage 1, 11-22.

McEwen, B.F. (1992) in "Electron Tomography", J. Frank ed., Plenum Press, New York, pp 281-311.