Ribosome (Eukaryotic) -- Small 40S Subunit

Subject:

Mammalian 40S ribosomal subunit with bound EIF-3 (eukaryotic initiation factor 3)

View:

Shaded surface representation of 3D volume

SPIDER/WEB users:

3d volume ( av.=.353, sigma=.049)

PJ ST image ( viewing angle = 180-deg., Threshold = .4325)

Specimen source:

Rabbit reticulocytes

Specimen preparation:

Native 40s subunits were prepared according to Lutsch et al. (1986) and examined by conventional transmission electron microscopy with double-carbon, negative staining methods, using uranyl acetate as contrasting agent.

Electron microscopy:

The reconstruction was calculated from 50-deg tilt data, using SECReT, the random conical reconstruction method of Radermacher. Minimal-dose (1000 el/nm**2) methods were used.

Magnification:

49,000 X. Pixel size = .51 nm.

Image processing:

see Radermacher (1988)

References:

Lutsch, G., Benndorf, R., Westermann, P., Bommer, U.-A., and Bielka, H. (1986) Structure and location of initiation factor eIF-3 within native small ribosomal subunits from eukaryotes. Eur. J. Cell Biol. 40, 257-265.

Radermacher,M. (1988) Three-dimensional reconstruction of single particles from random conical tilt series. J. El. Microsc. Tech. 9:359-394.

Srivastava, S., Verschoor, A., and Frank, J. (1992) Eukaryotic initiation factor 3 does not prevent association through physical blockage of the ribosomal subunit-subunit interface. J. Mol. Biol. 226:301-304.

Verschoor, A., Srivastava, Radermacher, M., Frank, J., Traut, R.R., Stoffler-Meilicke, M., and Glitz, D. (1993) Functional site determinations in three dimensions on eukaryotic and eubacterial ribosomes. In, (K.H. Nierhaus et al., eds.) The Translational Apparatus. New York, Plenum Press, 746 pp.