RIBOSOME (70S) at 25Å

Subject:

Ribosome (70S)/ice embedded

View:

Shaded surface representations in six(6) views related by rotation around vertical axis. The views correspond to those in Fig. 4 of Frank et al. (1995b).

SPIDER/WEB users: Threshold = 5.168016

Specimen source:

Escherichia coli.

Specimen preparation:

embedment in vitreous ice.

Electron microscopy:

Micrographs of untilted specimen grids. EM = Zeis Em912 using zero-loss energy filtering (14eV), Defocus settings 2.0 and 2.5 microns.

Magnification:

52,000X. Pixel size = .38 nm.

Image processing:

Orientation of 4300 Ribosome projections identified by 3D projection matching using low resolution reference. (Penczek et al., 1994). Reconstruction (SIRT) simultaneously performed CTF correction (Zhu et al. submitted).

References:

Frank, J., Zhu, J., Penczek, P., Li, Y., Srivastava, S., Verschoor, A., Radermacher, M., Grassucci, R., Lato, R.K., and Agrawal, R.K. (1995a), Nature 376 : 441-444.

Frank, J., Verschoor, A., Li, Y., Zhu, J., Lato, R.K., Radermacher, M., Penczek, P., Grassucci, R., and Agrawal, R.K. (1995b), Biochem. Cell Biol. 73 : 757-765.

Penczek, P., Grassucci, R., and Frank, J. (1994) Ultramicroscopy 53 : 251-270.

Model of translation: This is an interpretation of the 70s reconstruction in terms of