RIBOSOME (70S) at 45Å

Subject:

Ribosome (70S)/ice embedded

View:

Shaded representations in four views related by rotation around vertical axis. The views correspond to those in Fig. 5 of Penczek et al. (1992).

SPIDER/WEB users: Threshold = 5.168016

Specimen source:

Escherichia coli.

Specimen preparation:

embedment in vitreous ice.

Electron microscopy:

Data collection according to random-conical scheme described in Radermacher et al. (1987). Tilt angle 50 degres. E = Philips 420.

Magnification:

36,000X. Pixel size = .56 nm.

Image processing:

Three independent random-conical reconstructions were computed from particles lying in 3 distinct views. After determining the relative angles, the three projec- tion sets (total of 300) were combined in a merged reconstruction. See Penczek et al (1992) for details.

References:

Frank, J., Penczek, P., Grassucci, R., and Srivastava, S. (1991), J. Cell Biol. 115, 597-605.

Penczek, P., Radermacher, M., and Frank, J. (1992) Ultramicroscopy 40, 33-53.

Radermacher, M., Wagenknecht, T., Verschoor, A., and Frank, J. (1987), J. Microscopy 146, 113-136

These are four different views of the 70S ribosome of E. coli:

(a): Side view, showing the 30S and 50S subunits partially separated by a gap (interface gap).
(b): View of ribosome created by 40-degree rotation from (a) around vertical axis, showing the cytoplasmic side of the 30S subunit.
(c): View of ribosome created by 80-degree rotation from (a) around vertical axis, showing the cytoplasmic side of the 30S subunit ("Lake view").
(d): View of ribosome created by -110-degree rotation from (a) around vertical axis, showing the cytoplasmic side of the 50S subunit.