Ribosome (80S)

Subject:

Mammalian 80S ribosome/negative staining

View:

Shaded surface representation of 3D volume

SPIDER/WEB users: 3d volume ( av. = .008, sigma = .125). PJ ST image ( viewing angle = 180-deg, Threshold = .2)

Specimen source:

Rabbit reticulocytes

Specimen preparation:

Monomeric ribosomes were prepared for conventional transmission electron microscopy by double-carbon, negative staining methods, using uranyl acetate as contrasting agent.

Electron microscopy:

The reconstruction was calculated from 50-deg tilt data, using SECReT, the random conical reconstruction method of Radermacher and coworkers. Minimal-dose (1000 el/nm**2) methods were used.

Magnification:

49,000 X. Pixel size = .51 nm.

Image processing:

see Radermacher (1988)

References:

Frank, J., Verschoor, A., Radermacher, M., and Wagenknecht, T. (1990) Morphologies of eubacterial and eucaryotic ribosomes as determined by three-dimensional electron microscopy. In, (Hill, W.E. et al., eds.) The Ribosome: Structure, Function, and Genetics. Am. Soc. Microbiol., Washington, D.C. pp. 107-113.

Radermacher, M. (1988) Three-dimensional reconstruction of single particles from random conical tilt series. J. El. Microsc. Tech. 9:359-394.

Verschoor, A. and Frank, J. (1990) Three-dimensional structure of the mammalian cytoplasmic ribosome. J. Mol. Biol. 214:737-749.