ryanodine receptor reconstruction
Subject:
- Calcium release channel (ryanodine receptor).
View:
- Shaded surface representation of 3D volume.
Specimen source:
- Rabbit skeletal muscle.
Specimen preparation:
- The channels were prepared for conventional transmission
electron microscopy by a negative staining technique in
which the specimen and stain become sandwiched between
two thin layers of carbon.
Electron microscopy:
- The reconstruction was determined from microgaphs of
tilted specimen (tilt angle = 50 deg) using the random
conical method of Radermacher. Minimal dose
techniques were used (1000 electrons/nm**2)
Magnification:
- 36,000X.Pixel size = .69 nm.
Image processing:
- see Radermacher (1988).
References:
- Wagenknecht, T., Grassucci, R., Frank, J., Saito, A.,
Inui, M., and Fleischer,S. (1989) Three-dimensional
architecture of the calcium channel/foot structure of
sarcoplasmic reticulum. Nature 338:167-170.
Radermacher, M. (1988) Three-dimensional reconstruction
of single particles from random conical tilt series.
J. Electron Microscopy Technique 9:359-394.
View of a shaded surface representation of a 3D volume of
the calcium Release Channel:
Image