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MANUAL OF SPIDER OPERATIONS

Use the Cross-Reference Index to find operation for a desired task. A || symbol indicates the operation contains OMP parallel directives. A ||MPI symbol indicates the operation contains both OMP and MPI parallel directives. See also: SPIDER's Pocket Calculator

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@ Invoke a procedure
@@ Invoke a procedure without new namespace
AC Auto Correlation ||
AC MS Auto Correlation - Masked and normalized ||
AC MSS Self Correlation - Masked and normalized ||
AC N Auto Correlation - Normalized ||
AC NS Self Correlation - Normalized ||
AC S Self Correlation ||
AD Add images
AD F Add two images with factors
AD R Add two images with ratio
AD S Add Series of images
AP C Alignment - multi-reference, rotation and Classification ||
AP I Alignment - Initalize Rings File ||
AP MS Alignment - multi-reference, shift alignment ||
AP RA Alignment - Reference-free rotational alignment ||
AP REF Alignment - multi-reference, rotation & shift ||*
AP SA Alignment - Reference free, Shift ||
AP SCC Alignment - multi-reference, translation alignment ||
AP SH Alignment - multi-reference, exhaustive rotation & shift ||*
AP SHC Alignment - multi-reference, exhaustive rotation & shift ||*
AP SR Alignment - reference free, Shift & Rotation ||
AP TOOL Alignment - Tool for evaluating parameters ||
AR Arithmetic operation
AR IF Arithmetic operation on image - IF allowed
AR SCA Arithmetic scaling of image
AS Average images - find variance
AS AD Average images - add to an existing average
AS F Average images - find statistics & Q factor map ||
AS R Average images - find & save variance, better
AT IT Automatic particle picking - intermediate program.
AT PK Automatic particle picking - peak search
AT SA Automatic particle picking - statistical parameters ||
AT WN Automatic particle selection

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BC Box Convolution
BL Create Blank image
BP 32F Back Projection - 3D, Sampled, Interpolated in Fourier space ||*
BP 3D Back Projection - 3D, using Euler angles ||
BP 3F Back Projection - 3D, Interpolated in Fourier space ||*
BP 3G Back Projection - 3D, Gridded in Fourier space ||
BP 3N Back Projection - 3D, Nearest neighbor Interpolated in Fourier space ||
BP CG Back Projection - 3D, Iterative, Conjugate Gradients ||*
BP CG 3 Back Projection - 3D, Sampled, Conjugate gradients ||*
BP R2 Back Projection - Weighting image series ||
BP RP Back Projection - 3D, iterative, with constraints ||*
BP S2 Back Projection - 2D, single tilt, iterative, constrained ||
BP W2 Back Projection - 2D, filtered, weighted ||

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CA S Correspondence Analysis - Solution
CA SM Correspondence Analysis - Show Map & Eigen Values
CA SMI Correspondence Analysis - Set Active/Inactive Images Coordinates
CA SR Correspondence Analysis - Reconstitute images
CA SRA Correspondence Analysis - Arbitrary image reconstitution
CA SRD Correspondence Analysis - Reconstitute Differential images
CA SRE Correspondence Analysis - Reconstitution of eigenimages
CA SRI Correspondence Analysis - Reconstitute Importance images
CA VIS Correspondence Analysis - Create Visual map
CC Cross Correlation ||
CC C Cross Correlation Coefficient
CC H Cross Correlation - Phase ||
CC MS Cross Correlation - Masked and normalized ||
CC N Cross Correlation - Normalized ||
CC P Cross Correlation - Polar ||
CE AD Contrast Enhancement - Anisotropic Diffusion
CE FIT Contrast Enhancement - FIT the histogram
CE FREI Contrast Enhancement - Frei & Chen
CE G Contrast Enhancement - Gradient
CE GNC Contrast Enhancement - Graduated Non Convex restoration
CE HARALICK Contrast Enhancement - Haralick
CE HI Contrast Enhancement - Histogram Equalization
CE HURST Contrast Enhancement - Hurst
CE LAH Contrast Enhancement - Local Area Histogram
CE LAP Contrast Enhancement - Laplacian
CE MAX Contrast Enhancement - Maximum
CE MED Contrast Enhancement - Median Filtering
CE MET Contrast Enhancement - Maximum Entropy Threshold
CE MIN Contrast Enhancement - Minimum
CE OD Contrast Enhancement - adjust Optical Densities
CE OR Contrast Enhancement - Local Orientation
CE PREWITT Contrast Enhancement - Prewitt
CE RAN Contrast Enhancement - Range
CE RIDGE Contrast Enhancement - Ridge Following
CE SOBEL Contrast Enhancement - Sobel
CE ST Contrast Enhancement - Stretching
CE TOP Contrast Enhancement - Top Hat
CE VAR Contrast Enhancement - Variance
CE VS Contrast Enhancement - Variance smoothing
CE WATER Contrast Enhancement - Watershed Segmentation
CG Center of Gravity
CG PH Center of Gravity - Phase approximation ||
CG SYM Center of Object - Quasisymmetry Approximation ||
CI Confidence Interval
CL CLA Classification - Diday's Clustering
CL HC Classification - Hierarchical Clustering
CL HD Classification - Hierarchical, get number of classes
CL HE Classification - Hierarchical, create cluster selection files
CL KM Classification - K Means clustering ||
CN Convolution ||
CN N Convolution - Normalized ||
CO Contour into Postscript file
CP Copy SPIDER file to SPIDER file
CP FROM ASCII Copy ASCII text file to SPIDER file
CP FROM CCP4 Copy CCP4 format File to SPIDER file
CP FROM EMI Copy EMISPEC format file to SPIDER file
CP FROM MRC Copy Old MRC format file to SPIDER file
CP FROM NIKON Copy Nikon Coolscan format file to SPIDER file
CP FROM PDB Copy PDB file to SPIDER format
CP FROM RAW Copy Raw Image to SPIDER file
CP FROM TERM Copy Terminal input to SPIDER image/volume
CP FROM VAX Copy VAX SPIDER file to a Unix SPIDER file
CP FROM XP Copy X-PLOR ASCII file to SPIDER file
CP I Copy SPIDER file to Indexed SPIDER stack
CP TO ASCII Copy SPIDER file to ASCII text format
CP TO BRIX Copy SPIDER volume to BRIX file for "O" input
CP TO CCP4 Copy SPIDER file To CCP4 format file
CP TO MRC Copy SPIDER file To MRC format file
CP TO OPEND Copy SPIDER file To Opposite Byte Endian
CP TO POST Copy SPIDER image to a POSTscript image
CP TO RAW Copy SPIDER file to a 8-bit raw image
CP TO TIFF Copy SPIDER file to a TIFF format
CP TO XPLOR Copy SPIDER volume to X-PLOR format
CV REPL Projection onto Convex sets - Replace projector
CYCLE Starts next loop iteration (Scripting operation)

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DC Decimate image/volume
DC S Decimate image - Sum neighboring pixels
DE Delete a file
DE A Delete file series
DF Density Foldover
DI Dilate a binary image/volume
DI NF Dilate an object - do not fuse objects
DN AD Denoise - Anisotropic Diffusion
DN MED Denoise - Median Filtering
DN MS Denoise - Mean Shift
DO Start of DO loop (Scripting operation)
DOC AND "And" two Document files
DOC BOOT Select Document file entries - randomly, with replacement
DOC COMBINE Combine series of Document files.
DOC CREATE Create Document file
DOC KEY Re-Key Document file key numbers
DOC MERGE Merge two Document files
DOC MIRROR Mirror a Document file
DOC OLD Copy new format Document file to Old format
DOC RAN Randomly select Document file entries
DOC REN Renumber Document file keys
DOC SHUFFLE Shuffle Document file
DOC SORT Sort Document file
DOC SPLIT Split Document file into two Document files
DOC STAT Get Statistics from column in Document file
DOC SUBTRACT Subtract two Document files
DR DIFF Density Reference - Error & Difference between two volumes
DR ERR Density Reference - Error between two volumes
DU Dust removal
DU V Dust removal - specify value for removed pixels

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EC CL Extract Connected Clusters
EC CLOSE Extract Connected Clusters - merge close clusters
EC FILTER Extract Connected Clusters - Filter
EC SELECT Extract Connected Clusters - Selection
EC STAT Extract Connected Clusters - Statistics
ED Edge Detection
ELSE Conditional execution of operations (Scripting operation)
ELSEIF_THEN Conditional execution of operations (Scripting operation)
EN End SPIDER session
EN D End SPIDER session - Delete Results file
ENDDO End of DO loop (Scripting operation)
EP Error Probability
EP TM T test - Each Point
EP TT T test
ER Erode a binary image/volume
ER DOC Erode binary image/volume - Save UEP in doc file.
ER EDM Create Euclidean Distance Map
ER SK Erode a binary image to make skeleton
ER WA Erode image to a "watershed"
EV Set system environmental variable
EXIT Ends execution of current operation loop (Scripting operation)

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

FC File Contouring
FC E File Contouring - External contour levels
FD Filter according to a Document file ||
FF Fourier Filter
FF PLOT Fourier Filter - design filter
FI File Information - List and retrieve file header value(s) by location
FI A File Information - Multiple files
FI H File Information - Retrieve file Header values by name
FI N File Information - Do not halt if file not found
FI X File Information - Retrieve file header value(s) by location
FP Interpolate/Resize using Fourier transform ||
FQ Filter - Quick Fourier, High, low pass, etc. ||
FQ NP Filter - Quick Fourier, High, low pass, etc, No Padding ||
FR Read (sets) a local string (symbolic) variable
FR F Read (sets) global symbolic (string) variables from a file
FR G Read (sets) a global string (symbolic) variable
FR GS Sets global string variable using current string values
FR L Read (sets) a local string (symbolic) variable
FR LS Sets local string variable using current string values
FR N Read (sets) next global symbolic (string) variable from file
FS Find Statistics
FS M Find Statistics - within Mask
FT Fourier Transform ||
FT R Fourier Transform Replace
FV Find threshold for Volume

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GOTO GOTO (Scripting operation)
GP Get Pixel value
GR Graph an image row - in Results file
GR T Graph an image row - on Terminal

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

HD Histogram of Document file column - output in Results file
HD D Histogram of Document file column - output in Document file.
HD R Histogram of Document file column - output in Results file, use specified range
HF Helical Fourier
HI Histogram - output in Results file
HI D Histogram - output in Document file
HI DR Histogram - output in Document file, use specified Range
HI E Histogram - to determine entropy
HI JOINT Histogram - Joint, to determine mutual shared information
HI M Histogram - within Masked area
HI R HIstogram - output in Results file, use specified range.
HI T Histogram - output on Terminal
HI TR Histogram - output on Terminal, use specified range

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IA Interface Area
IF Conditional expression execution (Scripting operation)
IF_GOTO Conditional jump (Scripting operation)
IF_THEN Conditional execution of operations (Scripting operation)
IN Insert - image into a larger existing image
IN S Insert - image and contrast Stretch
IP Interpolate/Resize using bilinear/trilinear interpolation
IP FS Interpolate/Resize - using Fourier based bicubic spline ||
IP FT Interpolate/Resize - using Fourier Transform ||
IP T Interpolate/Resize - using Triangular interpolation
IQ DIR Inquire if a directory exists
IQ FI Inquire if a file exists
IQ GONE Wait until a file is gone
IQ PID Inquire current Process ID
IQ REG Inquire if a Register variable's value is correct
IQ SYNC Wait until a file exists
IQ SYNC D Wait until a file exists then delete it
IQ VER Inquire number of current SPIDER version
IQ WO Inquire values within raw files

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LA Add text label below an image
LA L Put text label inside an image
LB# Label for jump operation or end of DO-loop (Scripting operation)
LD List Document file
LD T List Document file - on Terminal
LI List image values - in Results file
LI D List image values - in Document file
LI T List image values - on terminal
LI X List image values - in register variables
LO LOcate motif inside a search volume
LO I Initialize files for LOcating motif inside volume
LO LSD Prepare LSD volume for LOcating motif inside volume

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

MA Mask - image/volume with a circular mask
MA L Mask - line across image
MA X Mask - image in X direction
MA Y Mask - image in Y direction
MA Z Mask - image in Z direction
MD Set Modes or options for operations
MK Marker based alignment - Single tilted images
MK 3 Marker based alignment - double tilted images
MM Mask Multiplication
MM C Mask Multiplication - Continuous
MN Montage of Images
MN S Montage of images - with individual Scaling
MO Model image creation
MO 3 Model volume creation
MR Mirror image or volume
MS Make empty inline Stack
MS 3 Make empty inline volume Stack
MS F Make empty inline Stack for Fourier images/volumes
MS I Make empty indexed Stack
MS IF Make empty Indexed Stack for Fourier files
MU Multiply two or more, real or complex images
MU D Divide real or complex files
MU M Multiply 1st complex file by 2nd conjugated file
MU O Multiply with arithmetic OR
MX Maximum of two images/volumes
MY User supplied subroutines
MY FL Flush output to results file

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NC New data/project file extension
NEG Negate - invert contrast of image
NEG A Negate - invert contrast of image & preserve average

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OD Optical Density conversion
OP Orientation of Projections in terms of Eulerian angles.
OR 3A ORientation search - 3D, Eulerian angles, arbitrary center ||
OR 3Q ORientation search - 3D, Eulerian angles ||
OR A ORientation search - 2D, using self-correlation ||
OR Q ORientation search - 2D, multireference, using self-correlation ||
OR R ORientation search - 2D, rotation, remapping into polar coordinates ||
OR SH ORientation search - 2D, rotation & shift, uses polar coords. ||

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

PA Patch image into a larger existing image/volume
PD Pad Image/volume with borders
PDB CG3 PDB - Calculate center of gravity
PDB IF PDB - Find information
PDB PTS PDB - Put coordinates in SPIDER document file.
PDB RT3 PDB - Rotates PDB file in 3D space
PDB RT3A PDB - Rotate PDB file by Euler angles around arbitrary center
PDB RT3L PDB - Rotate PDB file around line
PDB SH SHift coordinates in a PDB file
PDB STP PDB - Create PDB file from coordinate list
PF Profile an image row in a Postscript graph
PI REG Output Register variable value on pipe
PJ 3 Project - 3D volume for single projection
PJ 3F Project - 3D volume using Eulerian angles ||
PJ 3G Project - 3D volume using Gridding by Eulerian angles ||
PJ 3Q Project - 3D volume using Eulerian angles ||
PJ 3Q RR Project - 3D volume using Eulerian angles & create rings file ||
PJ AT Project - 3D volume using exponential Attenuation
PJ COL Projected Surface - Coloring
PJ CYL Project - 3D volume onto Cylinder
PJ SHAD Projected Surface - cosine Shading
PJ ST Projected Surface - Creation
PK Peak search
PK 3 Peak search - 3D
PK 3D Peak search - 3D, output to Document file
PK 3R Peak search - 3D, Restricted neighborhood, output to Document file
PK C Peak search - Center of gravity
PK D Peak search - output to Document file
PK DC Peak search - Center of gravity, output to Document file
PK DR Peak search - Restricted neighborhood, output to Document file
PK M Peak search - Maximum
PL Plot Document file data into Postscript file
PL FIT Fit Polynomial to Document file data
PL HI Plot Histogram - from Document file
PO Convert an image to polar coordinates representation.
PP Put Points in image - from terminal
PP L Put Points in image - from Document file Listing
PP LL Put Lines in image - from Document file Listing
PP P Put Points in image - from Document file, alters neighbors
PP V Put height image into Volume
PS Pick Slice - Y (xz planes)
PS A Pick Slice - Arbitrary direction
PS X Pick Slice - X, (yz planes)
PS Z Pick Slice - Z (xy planes)
PT Create Pattern or mask image file
PW Amplitudes of Fourier transform
PW L Power spectrum - Log10

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RA Create Ramp image
RB 32F Rotate, Shift, Sampled, Back Projection in Fourier space |
RB 3F Rotate, Shift, Back Projection in Fourier space |
RC Real Convolution ||
RE Return from procedure
RF Fourier Ring correlation Factor ||
RF 3 Phase Residual & Fourier shell correlation, 3D ||
RF 3SN 3D Spectral SNR of a 3D reconstruction ||
RF SN Spectral SNR of images ||
RM Radon transform suite of programs.
RM 2DN Radon Transform - 2D
RM 3DN Radon Transform - 3D
RM COMPLN Radon Transform - 2D, Complete
RM FILTER Radon Transform - Filter
RM FTINVN Radon Transform - Inverse Fourier transform
RM FTN Radon Transform - Fourier transform
RM INV2N Radon Transform - Computes inverse (Fourier-)Radon transform
RM INV3N Radon Transform - Computes inverse 3D (Fourier-)Radon transform
RM MAKE3 Radon Transform - Create blank 3D Radon (or Polar Fourier) Transf.
RM RESET Radon Transform - Clear counter and sigma
RM SUM3N Radon Transform - Sum/average 2D Into 3D Fourier Radon transform
RO Rotational average - single line output
RO I Rotational average - 2D Image output
ROT ROTate image/volume around center by specified angle(s)
ROT A ROTate around arbitrary center
ROT L ROTate volume around specified line
RP Replace Pixel value
RR Read (Set) Register variables
RR C Read Register variables - Copy from global
RR G Read Register variables - Global
RR S Read Register variable - from list of variables
RT 90 RoTate - image or volume, by 90, 180, or 270 degrees
RT SF Rotate and Shift - image, Fourier based spline interpolation ||
RT SQ Rotate and Shift - image, Quadratic interpolation ||
RTD SF Rotate and Shift - image, Fourier based spline interpolation ||
RTD SQ Rotate and Shift - image, Quadratic interpolation ||

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SA Sum Alignment
SA 3 Sum Alignment in 3D
SA E Sum Eulerian angles
SA P Sum Alignment - Positive rotation and shift
SD Save Document
SD C Save Document - from CA S (_IMC) file
SD E Save Document - End
SD IC Save Document - In Core
SD IC COPY Save Document - In Core, Copy
SD IC NEW Save Document - In Core, create New storage array
SD SHUFFLE Shuffle Document file
SE Seed fill an image
SH Shift - using bilinear/trilinear interpolation ||
SH F Shift - using Fourier interpolation ||
SK Stack images into volume
SK R Replace a slice in volume
SL Create slice through image series
SN RB SNR Butterworth
SN RF SNR Filter
SO Surface extraction
SO C Surface Comparision
SP Find info on diffraction pattern spots
SQ Square image values
SQRT Square root of image/volume
ST Set values in image header
ST H Set values in image/volume Header by name
SU Subtract images/volumes
SY DOC Create Symmetry Document file
SZ Squeezes (Shears) an Image

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

TF Transfer Function - Defocus dependent
TF C Transfer Function - Complex 2D
TF C3 Transfer Function - Complex 3D
TF COR Transfer Function - CTF correction and back transfom
TF CRF Transfer Function - Determine Defocus & ACR
TF CT Transfer Function - phase flipping, Complex, Binary
TF CT3 Transfer Function - Complex, Binary 3D
TF CTS Transfer Function - 2D & 3D CTF correction
TF D Transfer Function - Display
TF DDF Transfer Function - Determine Defocus & amplitude contrast
TF DEV Transfer Function - Determine Envelope function
TF DNS Transfer Function - Determine and delete Noise background
TF ED Transfer Function - Estimation of CTF parameters
TF L Transfer Function - Send to document file
TF LM4 Transfer Function - Estimate image formation parameters
TF RCTF Transfer Function - Rotational average?
TF SIM Transfer Function - Simulate an electron microscope image
TF SNR Transfer Function - 3D CTF correction, Freq. dependent snr
TH Threshold - Set pixels above/below Threshold to Threshold
TH C Threshold - Change all pixels having a specific value in image
TH F Threshold - Set pixels above/below Threshold to Fixed value
TH M Threshold - Create Mask based on Threshold
TM Elapsed Time
TP Three dimensional Plot of image values
TT Sets image Title
TT COPY Copies image Title

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

UD Unsave Document
UD E Unsave Document - End
UD FIND Unsave Document - Find value
UD FIND E Unsave Document - Fine End
UD IC Unsave Document - In Core
UD ICE Unsave Document - In Core, End
UD MAX Unsave Document - get Max/Min from document column
UD N Unsave Document - get key Numbers
UD NEXT Unsave Document - Next line
UD NEXT E Unsave Document - End
UD S Unsave Document - Sequential search

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

VA 3Q Bootstrapping method for variance estimation
VA 3R Variance estimation
VA F Variance of reconstruction in Fourier space as function of frequency ||
VAR Variable Assignment
VM System call
VO EA Vector Operation - Create Evenly Spaced Angles Document file
VO EPT Vector Operation - Euler angle point rotation
VO IA Vector Operation - Find Included Angle
VO MD Vector Operation - angular Document file from 'AP MD' output
VO MQ Vector Operation - create selection files from 'AP' output
VO NEA Vector Operation - Create More Uniformly Spaced Angles
VO RA Vector Operation - Rotate Angular document file
VO RAS Vector Operation - Rotate Angular document file & Set angle

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

WA Warp an image/volume
WI Window image
WI B Window image - use specified Background
WV Window averaging
WV P Window averaging - over Patches
WV S Window averaging - Sequential document search
XM MA Create Xmipp selfile

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