HOME GUIDE OPERATIONS DOCS FAQ TECHNIQUES FORMATS INSTALL NEW TIPS WEB LINKS WADSWORTH

MANUAL OF SPIDER OPERATIONS

Use the Cross-Reference Index to find operation for a desired task. A || symbol indicates the operation contains OMP parallel directives. A ||* symbol indicates the operation contains both OMP and MPI parallel directives. See also: SPIDER's Pocket Calculator

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

AC Auto Correlation ||
AC MS Auto Correlation - Masked and normalized ||
AC MSS Self Correlation - Masked and normalized ||
AC N Auto Correlation - Normalized ||
AC NS Self Correlation - Normalized ||
AC S Self Correlation ||
AD Add images
AD F Add two images with factors
AD R Add two images with ratio
AD S Add Series of images
AP CA Alignment - Classification and Alignment
AP CM Alignment - Classification and Alignment
AP I Alignment - Initalize Rings File ||
AP MS Alignment - multi-reference, shift alignment ||
AP RA Alignment - Reference-free rotational alignment ||
AP REF Alignment - multi-reference, rotation & shift ||*
AP SA Alignment - Shift ||
AP SCC Alignment - multi-reference, translation alignment ||
AP SH Alignment - multi-reference, exhaustive rotation & shift ||*
AP SR Alignment - reference free, Shift & Rotation ||
AR Arithmetic operation
AR IF Arithmetic operation - IF allowed
AR SCA Arithmetic scaling of image
AS Average images - find variance
AS AD Average images - add to an existing average
AS F Average images - find statistics & Q factor map ||
AS R Average images - find & save variance, better
AT IT Automatic particle picking - intermediate program.
AT PK Automatic particle picking - peak search
AT SA Automatic particle picking - statistical parameters ||
AT WN Automatic particle selection
return to top

BC Box Convolution
BL Create Blank image
BP 32F Back Projection - 3D, Odd-even, interpolated in Fourier space ||*
BP 3D Back Projection - 3D, using Euler angles ||
BP 3F Back Projection - 3D, interpolated, in Fourier space ||*
BP CG Back Projection - 3D, conjugate gradients, ||*
BP R2 Back Projection - 2D, weighting of image series ||
BP RP Back Projection - 3D, iterative, with constraints, ||*
BP S2 Back Projection - 2D, single tilt iterative, constrained ||
BP W2 Back Projection - 2D, filtered, weighted ||
BPD 32F Back Projection - 3D, Sampled, Interpolated in Fourier space ||*
BPD 3F Back Projection - 3D, Interpolated in Fourier space ||*
return to top

CA S Correspondence Analysis - Solution
CA SM Correspondence Analysis - Show Map & Eigen Values
CA SMI Correspondence Analysis - Set Active/Inactive Images Coordinates
CA SR Correspondence Analysis - Reconstitute images
CA SRA Correspondence Analysis - Arbitrary image reconstitution
CA SRD Correspondence Analysis - Reconstitute Differential images
CA SRE Correspondence Analysis - Reconstitution of eigenimages
CA SRI Correspondence Analysis - Reconstitute Importance images
CA VIS Correspondence Analysis - Create Visual map
CC Cross Correlation ||
CC C Cross Correlation Coefficient
CC MS Cross Correlation - Masked and normalized ||
CC N Cross Correlation - Normalized ||
CC P Cross Correlation - Polar ||
CE AD Contrast Enhancement - Anisotropic Diffusion
CE FIT Contrast Enhancement - FIT the histogram
CE FREI Contrast Enhancement - Frei & Chen
CE G Contrast Enhancement - Gradient
CE GNC Contrast Enhancement - Graduated Non Convex restoration
CE HARALICK Contrast Enhancement - Haralick
CE HI Contrast Enhancement - Histogram Equalization
CE HURST Contrast Enhancement - Hurst
CE LAH Contrast Enhancement - Local Area Histogram
CE LAP Contrast Enhancement - Laplacian
CE MAX Contrast Enhancement - Maximum
CE MED Contrast Enhancement - Median Filtering
CE MET Contrast Enhancement - Maximum Entropy Threshold
CE MIN Contrast Enhancement - Minimum
CE OD Contrast Enhancement - adjust Optical Densities
CE OR Contrast Enhancement - Local Orientation
CE PREWITT Contrast Enhancement - Prewitt
CE RAN Contrast Enhancement - Range
CE RIDGE Contrast Enhancement - Ridge Following
CE SOBEL Contrast Enhancement - Sobel
CE ST Contrast Enhancement - Stretching
CE TOP Contrast Enhancement - Top Hat
CE VAR Contrast Enhancement - Variance
CE VS Contrast Enhancement - Variance smoothing
CE WATER Contrast Enhancement - Watershed Segmentation
CG Center of Gravity
CG 3 Center of Gravity - Radius of gyration
CG 3S Center of Gravity - Solid body option
CG PH Center of Gravity - Phase approximation ||
CI Confidence Interval
CL CLA Classification - Clustering
CL HC Classification - Hierarchical Clustering
CL HD Classification - Hierarchical clustering, calculate classes
CL HE Classification - Hierarchical clustering, create docfiles
CL KM Classification - K Means clustering ||
CN Convolution ||
CN N Convolution - Normalized ||
CO Contour into Postscript file
CP Copy SPIDER file to SPIDER file
CP FROM ASCII Copy ASCII text file to SPIDER file
CP FROM CCP4 Copy CCP4 format File to SPIDER file
CP FROM EMI Copy EMISPEC format file to SPIDER file
CP FROM MRC Copy MRC format file to SPIDER file
CP FROM NIKON Copy Nikon Coolscan format file to SPIDER file
CP FROM PDB Copy PDB file to SPIDER format
CP FROM PE Copy Perkin Elmer disk file to SPIDER format
CP FROM RAW Copy Raw Image to SPIDER file
CP FROM TERM Copy Terminal input to SPIDER file
CP FROM VAX Copy VAX SPIDER file to a Unix SPIDER file
CP FROM XP Copy X-PLOR ASCII file to SPIDER file
CP I Copy SPIDER file to Indexed SPIDER stack
CP TO ASCII Copy SPIDER file to ASCII text format
CP TO BRIX Copy SPIDER volume to BRIX file for "O" input
CP TO CCP4 Copy SPIDER file TO CCP4 format file
CP TO MRC Copy SPIDER file TO MRC format file
CP TO OPEND Copy SPIDER file TO Opposite Endedness
CP TO POST Copy SPIDER image to a POSTscript image
CP TO RAW Copy SPIDER file to a 8-bit raw image
CP TO SGI Copy SPIDER file to SGI format
CP TO TIFF Copy SPIDER file to a TIFF format
CP TO VV Copy SPIDER volume to Voxel View format
CP TO XPLOR Copy SPIDER volume to X-PLOR format
CV REPL Projection onto Convex sets - Replace projector
CYCLE Starts next loop iteration
return to top

DC Decimate image
DC S Decimate image - Sum neighbouring pixels
DE Delete a file
DE A Delete file series
DF Density Foldover
DI Dilate a binary image/volume
DI NF Dilate an object - do not fuse objects
DO Start of DO loop
DOC AND "And" two Document files
DOC BOOT Select Document file entries - randomly, with replacement
DOC COMBINE Combine series of Document files.
DOC CREATE Create Document file
DOC KEY Re-Key Document file key numbers
DOC MERGE Merge two Document files
DOC MIRROR Mirror a Document file
DOC OLD Copy new format Document file to Old format
DOC RAN Randomly select Document file entries
DOC REN Renumber Document file keys
DOC SORT Sort Document file
DOC SPLIT Split Document file into two Document files
DOC SUBTRACT Subtract two Document files
DR DIFF Density Reference - Error & Difference between two volumes
DR ERR Density Reference - Error between two volumes
DU Dust removal
DU V Dust removal - specify value for removed pixels
return to top

EC CL Extract Connected Clusters
EC CLOSE Extract Connected Clusters - merge close clusters
EC FILTER Extract Connected Clusters - Filter
EC SELECT Extract Connected Clusters - Selection
EC STAT Extract Connected Clusters - Statistics
ED Edge Detection
ELSE Conditional execution of operations
ELSEIF_THEN Conditional execution of operations
EN End SPIDER session
EN D End SPIDER session - Delete Results file
ENDDO End of DO loop
EP Error Probability
EP TM T test - Each Point
EP TT T test
ER Erode a binary image/volume
ER DOC Erode binary image/volume - Save UEP in doc file.
ER EDM Create Euclidean Distance Map
ER SK Erode a binary image to make skeleton
ER WA Erode image to a "watershed"
EV Set system environmental variable
EXIT Ends execution of current loop of operations
return to top

FC File Contouring
FC E File Contouring - External contour levels
FD Filter according to a Document file ||
FF Fourier Filter
FF PLOT Fourier Filter - design filter
FI File Information - place header value(s) in register variable
FI A File Information - Multiple files
FI N File Information - Do not halt if file not found
FI X File Information - place header values in registers
FP Fourier interpolation ||
FQ Filter - Quick Fourier, High, low pass, etc. ||
FQ NP Filter - Quick Fourier, High, low pass, etc, No Padding ||
FR Read (sets) a local symbolic parameter (string variable)
FR F Read (sets) global symbolic parameter (string variable) from a file
FR G Read (sets) a global symbolic parameter (string variable)
FR L Read (sets) a local symbolic parameter (string variable)
FR N Read (sets) next global symbolic parameter (string variable) from file
FS Find Statistics
FS M Find Statistics - within Mask
FT Fourier Transform ||
FT R Fourier Transform Replace
FV Find threshold for Volume
return to top

GOTO GOTO scripting command
GP Get Pixel value
GR Graph an image row - in Results file
GR T Graph an image row - on Terminal
return to top

HD Histogram of Document file column - output in Results file
HD D Histogram of Document file column - output in Document file.
HD R Histogram of Document file column - output in Results file, use specified range
HF Helical Fourier
HI Histogram - output in Results file
HI D Histogram - output in Document file
HI DR Histogram - output in Document file, use specified Range
HI E Histogram - to determine entropy
HI JOINT Histogram - Joint, to determine mutual shared information
HI M Histogram - within Masked area
HI R HIstogram - output in Results file, use specified range.
HI T Histogram - output on Terminal
HI TR Histogram - output on Terminal, use specified range
return to top

IA Interface Area
IF Conditional expression execution
IF_GOTO Conditional jump
IF_THEN Conditional execution of operations
IN Insert - image into a larger existing image
IN S Insert - image and contrast Stretch
IP Interpolate - Bilinear
IP T Interpolate - Triangular
IQ FI Inquire if a file exists
IQ GONE Wait until a file is gone
IQ PID Inquire current Process ID
IQ REG Inquire if a Register variable's value is correct
IQ SYNC Wait until a file exists
IQ WO Inquire values within raw files
return to top

LA Add text label below an image
LA L Put text label inside an image
LB# Label for jump operation or end of DO-loop
LD List Document file
LD T List Document file - on Terminal
LI List image values - in Results file
LI D List image values - in Document file
LI T List image values - on terminal
LI X List image values - in register variables
return to top

MA Mask - image using circular mask
MA L Mask - line across image
MA X Mask - image in X direction
MA Y Mask - image in Y direction
MA Z Mask - image in Z direction
MD Set Modes or options for operations
MK Marker based alignment - Single tilted images
MK 3 Marker based alignment - double tilted images
MM Mask Multiplication
MM C Mask Multiplication - Continuous
MN Montage of Images
MN S Montage of images - with individual Scaling
MO Model image creation
MO 3 Model volume creation
MR Mirror image or volume
MS Make empty inline Stack
MS 3 Make empty inline volume Stack
MS F Make empty inline Stack for Fourier images/volumes
MS I Make empty indexed Stack
MS IF Make empty Indexed Stack for Fourier files
MU Multiply two or more, real or complex images
MU D Divide real or complex files
MU M Multiply 1st complex file by 2nd conjugated file
MU O Multiply with arithmetic OR
MX Maximum of image/volume
MY User supplied subroutines
MY FL Flush output to results file
return to top

NC New data/project file extension
NEG Negate - invert contrast of image
NEG A Negate - invert contrast of image & preserve average
NK Shrink to threshold
NK M Shrink to mask
return to top

OD Optical Density conversion
OP Orientation of Projections in terms of Eulerian angles.
OR ORientation search - half circle used
OR 2 ORientation search - 2D ||
OR 2M ORientation search - 2D, multiple peaks ||
OR 3A ORientation search - 3D, Eulerian angles, arbitrary center ||
OR 3Q ORientation search - 3-D, Eulerian angles ||
OR A ORientation search - 2D, using self-correlation functions ||
OR F ORientation search - full circle used
OR FM ORientation search - Full circle, Find > one peak
OR M ORientation search - find More than one peak
OR MQ ORientation search - resamples into polar coords, checks mirror ||
OR NQ ORientation search - resamples into polar coords. ||
OR Q Multireference alignment - based on self-correlation functions ||
return to top

PA Patch image into a larger existing image/volume
PD Pad Image/volume with borders
PDB CG3 PDB - Calculate center of gravity
PDB IF PDB - Find information
PDB PTS PDB - Put coordinates in SPIDER document file.
PDB RT3 PDB - Rotates PDB file in 3D space
PDB RT3A PDB - Rotate PDB file by Euler angles around arbitrary center
PDB RT3L PDB - Rotate PDB file around line
PDB SH SHift coordinates in a PDB file
PDB STP PDB - Create PDB file from coordinate list
PF Profile an image row in a Postscript graph
PI REG Output Register variable value on pipe
PJ 3 Project - 3D volume
PJ 3O Project - 3D volume (older version)
PJ 3Q Project - 3D volume using Eulerian angles ||
PJ 3Q O Project - 3D volume using Eulerian angles (older version) ||
PJ 3Q RR Project - 3D volume using Eulerian angles & create rings file ||
PJ AT Project - 3D volume using exponential Attenuation
PJ COL Projected Surface - Coloring
PJ CYL Project - 3D volume onto Cylinder
PJ SHAD Projected Surface - cosine Shading
PJ ST Projected Surface - Creation
PK Peak search
PK 3 Peak search - 3D
PK 3D Peak search - 3D, output to Document file
PK 3R Peak search - 3D, Restricted neighborhood, output to Document file
PK C Peak search - Center of gravity
PK D Peak search - output to Document file
PK DC Peak search - Center of gravity, output to Document file
PK DR Peak search - Restricted neighborhood, output to Document file
PK M Peak search - Maximum
PL Plot Document file data into Postscript file
PL FIT Fit Polynomial to Document file data
PL HI Plot Histogram - from Document file
PO Convert an image to polar coordinates representation.
PP Put Points in image - from terminal
PP L Put Points in image - from Document file Listing
PP LL Put Lines in image - from Document file Listing
PP P Put Points in image - from Document file, alters neighbors
PP V Put height image into Volume
PS Pick Slice - Y (xz planes)
PS A Pick Slice - Arbitrary direction
PS X Pick Slice - X, (yz planes)
PS Z Pick Slice - Z, (xy planes)
PT Create Pattern or mask file
PW Amplitudes of Fourier transform
PW 2 Power spectrum
PW L Power spectrum - Log10
return to top

RA Create Ramp image
RB 32F Rotate, Shift, Sampled, Back Projection in Fourier space |
RB 3F Rotate, Shift, Back Projection in Fourier space |
RC Real Convolution ||
RE Return from procedure
RF R Factor ||
RF 3 Phase Residual & Fourier shell correlation, 3D ||
RF 3SN 3D Spectral SNR of a 3D reconstruction ||
RF SN Spectral SNR of images ||
RM Radon transform suite of programs.
RM 2DN Radon Transform - 2D
RM 3DN Radon Transform - 3D
RM COMPLN Radon Transform - 2D, Complete
RM FILTER Radon Transform - Filter
RM FTINVN Radon Transform - Inverse Fourier transform
RM FTN Radon Transform - Fourier transform
RM INV2N Radon Transform - Computes inverse (Fourier-)Radon transform
RM INV3N Radon Transform - Computes inverse 3D (Fourier-)Radon transform
RM MAKE3 Radon Transform - Create blank 3D Radon (or Polar Fourier) Transf.
RM RESET Radon Transform - Clear counter and sigma
RM SUM3N Radon Transform - Sum/average 2D Into 3D Fourier Radon transform
RO Rotational average - single line output
RO I Rotational average - 2D Image output
RP Replace Pixel value
RR Read Register variables
RR C Read Register variables - Copy from global
RR G Read Register variables - Global
RR S Read Register variable - from list of variables
RT RoTate - image or volume, use average as background
RT 3 RoTate - volume, slow
RT 3A RoTate - volume, arbitrary center, quadratic interpolation
RT 3AS RoTate - volume, arbitrary center, simple interpolation
RT 3D RoTate - volume, fast, quadratic interpolation
RT 3DS RoTate - volume, fast, simple interpolation
RT 3L RoTate - volume around an arbitrary line
RT 3LS RoTate - volume around arbitrary line, simple interpolation
RT 90 RoTate - image or volume, by 90, 180, or 270 degrees
RT B RoTate - image or volume, supply Background
RT C RoTate - image, around arbitrary center, for small files
RT M RoTate - image or volume, use minimum as background
RT SQ Rotate and Shift - image, Quadratic interpolation ||
RTD SQ Rotate and Shift - image, Quadratic interpolation ||
return to top

SA Sum Alignment
SA 3 Sum Alignment in 3D
SA E Sum Eulerian angles
SA P Sum Alignment - Positive rotation and shift
SD Save Document
SD C Save Document - from CA S (_IMC) file
SD E Save Document - End
SD IC Save Document - In Core
SD IC COPY Save Document - In Core, Copy
SD IC NEW Save Document - In Core, create New storage array
SD S Sort Document file according to one column
SD SHUFFLE Shuffle Document file
SE Seed fill an image
SH Shift - using bilinear interpolation ||
SH F Shift - using Fourier interpolation ||
SK Stack images into volume
SK R Replace a slice in volume
SL Create slice through image series
SN RB SNR Butterworth
SN RF SNR Filter
SO Surface extraction
SO C Surface Comparision
SP Find info on diffraction pattern spots
SQ Square image values
SQRT Square root of image/volume
ST Set values in image header
SU Subtract images/volumes
SY DOC Create Symmetry Document file
SZ Squeezes (Shears) an Image
return to top

TF Transfer Function - Defocus dependent
TF C Transfer Function - Complex
TF C3 Transfer Function - Complex 3D
TF CRF Transfer Function - Determine Defocus & Amplitude contrast ratio
TF CT Transfer Function - phase flipping, Complex, Binary
TF CT3 Transfer Function - Complex, Binary 3D
TF CTS Transfer Function - 2D & 3D CTF correction
TF CTS2 Transfer Function - 2D CTF correction
TF D Transfer Function - Display
TF DDF Transfer Function - Determine DeFocus & amplitude contrast
TF DEV Transfer Function - Determine Envelope function
TF DNS Transfer Function - Determine and delete Noise background
TF ED Transfer Function - Estimation of CTF parameters
TF L Transfer Function - Send to document file
TF LM4 Transfer Function - estimate image formation parameters
TF SIM Transfer Function - Simulate an electron microscope image
TF SNR Transfer Function - 3D CTF correction
TH Threshold - Set pixels above/below Threshold to Threshold
TH C Threshold - Change all pixels having a specific value in image
TH F Threshold - Set pixels above/below Threshold to Fixed value
TH M Threshold - Create Mask based on Threshold
TM Elapsed Time
TP Three dimensional Plot of image values
TT Sets image Title
TT COPY Copies image Title
return to top

UD Unsave Document
UD E Unsave Document - End
UD IC Unsave Document - In Core
UD ICE Unsave Document - In Core, End
UD N Unsave Document - get key Numbers
UD NEXT Unsave Document - Next line
UD NEXT E Unsave Document - End
UD S Unsave Document - Sequential search
return to top

VA F Variance of reconstruction in Fourier space as function of frequency ||
VM System call
VO CEUL Vector Operation - Combine Euler rotations
VO EA Vector Operation - Create Evenly Spaced Angles Document file
VO EPT Vector Operation - Euler angle point rotation
VO IA Vector Operation - Find Included Angle
VO MD Vector Operation - angular Document file from 'AP MD' output
VO MQ Vector Operation - create selection files from 'AP' output
VO NEA Vector Operation - Create More Uniformly Spaced Angles
VO RA Vector Operation - Rotate Angular document file
VO RAS Vector Operation - Rotate Angular document file & Set angle
return to top

WA Warp a volume
WI Window image
WI B Window image - use specified Background
WT Lattice indexing
WT TV Lattice Indexing - create filter file
WV Window averaging
WV P Window averaging - over Patches
WV S Window averaging - Sequential document search
return to top

© Copyright Notice /       Enquiries: spider@wadsworth.org

BACK TO SPIDER BACK TO WADSWORTH