Single Tilt Backprojection using PubSub
- Login to master node of cluster, where PubSub has been
installed and is running, e.g.:
rlogin patroon
- Access your working_dir, e.g.:
cd /usr1/user/bp
- Create input and output sub-directories, e.g.:
mkdir input output
- Copy all the necessary input data files, i.e.:
Image selection doc. file, Projection angles doc. file, and
Aligned projections to the input directory e.g.:
scp '/usr11/user/bp/angles.dat' input
scp '/usr11/user/bp/sel003.dat' input
scp '/usr11/user/bp/k35_rtsq_*.dat' input
- Copy bpw2_pub.spi procedure file to the working directory, e.g.:
scp -v '/usr8/spider/proc/bpw2_pub.spi' .
- Edit bpw2_pub.spi to set
necessary values for input parameters and input file names, e.g.
xedit bpw2.spi
The number of OMP threads should be 2, Number of partitions/nodes = 1...15, and
the dimensions are determined by input image dimensions.
- Copy a specific SPIDER executable to your current dir. (ensures that same SPIDER
executable is used throughout whole run), e.g.:
cp /us8/spider/bin/spider_linux_mpfftw.18.15.0 spider
- Start Master SPIDER process bpw2_pub.spi, e.g.
spider spi/dat bpw2_pub 0 &
- Master SPIDER refinement process will create and publish
new SPIDER jobs as necessary.
Source file: /usr8/spider/pubsub/bpw2_pub.html
Updated: 8/10/10
ArDean Leith