Skip to main content

You are here

Frank Maley, Ph.D.

  • Headshot portrait of Dr. Frank Maley.

    Frank Maley, Ph.D.

    • Research Scientist Emeritus

    • Ph.D., University of Wisconsin-Madison (1956)
    • Postdoctoral training: New York University Medical School

    frank.maley@health.ny.gov


Research Interests

Relationship of protein Structure to enzyme activity with respect to bacterial and human Thymidylate Synthases and Deoxycytidylate Deaminases using x-ray crystallography and site directed mutagenesis.

Developing systems for the high level expression of these proteins and examining the regulation of protein synthesis at the genomic and translational level.

Determining the intracellular location of Thymidylate Synthase and the effect that phosphorylation has on this process.

The role that an intron has on the expression of T4-Phage Thymidylate Synthase.

Defining the location and function of N-coupled oligosaccharides in glycoproteins using an endoglycosidase (Endo-H ) discovered in our laboratory.

Select Publications

Saxl RL, Maley GF, Hauer CR, Maccoll R, Changchien L, Maley F.
Significance of mutations on the structural perturbation of thymidylate synthase: implications for their involvement in subunit exchange.
Protein Sci.
(2007)
16
(7):
1439-48.
Almog R, Maley F, Maley GF, Maccoll R, Van Roey P.
Three-dimensional structure of the R115E mutant of T4-bacteriophage 2'-deoxycytidylate deaminase.
Biochemistry.
(2004)
43
(43):
13715-23.
West DK, Porter DC, Saxl RL, Maley F.
A trojan horse approach for silencing thymidylate synthase.
Biochemistry.
(2004)
43
(28):
9177-84.
Saxl RL, Reston J, Nie Z, Kalman TI, Maley F.
Modification of Escherichia coli thymidylate synthase at tyrosine-94 by 5-imidazolylpropynyl-2'-deoxyuridine 5'-monophosphate.
Biochemistry.
(2003)
42
(15):
4544-51.
Saxl RL, Changchien LM, Hardy LW, Maley F.
Parameters affecting the restoration of activity to inactive mutants of thymidylate synthase via subunit exchange: further evidence that thymidylate synthase is a half-of-the-sites activity enzyme.
Biochemistry.
(2001)
40
(17):
5275-82.