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Leslie E. Eisele, M.S.

Leslie E. Eisele, M.S.

Director, Biochemistry and Immunology Core
M.S., University at Albany (1985)

Research Interests

As Director of the Biochemistry Core facility I am responsible for the implementation and support of multiple technologies designed to characterize biomolecules and their interactions with one another.  The core houses equipment for purifying, quantifying, and characterization; particularly of biomolecular interactions in solution.  Whether we are measuring binding capabilities of neutralizing antibodies against a toxin, developing more sensitive fluorescence methods to identify environmentally significant molecules or probing quaternary structure and thermal stability of Mycobacterium tuberculosis proteins to better understand their role in virulence, my expertise lies in guiding scientists towards appropriate strategies to answer biophysical research questions.  Our instrumentation includes HPLC and UPLC, Analytical Ultracentrifugation, SEC-MALS (size exclusion chromatography multiangle laser light scattering), Circular Dichroism, Fluorescence Spectroscopy and Surface Plasmon Resonance.  These techniques are invaluable in determining the stability, size, conformation, and binding characteristics of biomolecules (proteins, DNA, low molecular weight ligands) for current biological research and essential for defining the normal and disease state for health related studies.

The goal is to provide easy access to technical instrumentation and specialized methods.  Training in concepts and use of the instruments is typically one-on-one, followed by supported work with scientists as they perform their experiments.

The Biochemistry Core was combined with Dr. Renjie Song’s Immunology Core in 2014.  Dr. Song is expert in flow cytometry, instrumentation used to assess the immunophenotype of cell types and specific cell receptor(s), ligand  binding, expression of surface and intracellular antigens, signal transduction events such as calcium flux, cell cycle progression based on analysis of DNA and RNA, apoptosis and cell sorting (including single cells).

Select Publications

Herrera C, Vance DJ, Eisele LE, Shoemaker CB, Mantis NJ.
Differential neutralizing activities of a single domain camelid antibody (VHH) specific for ricin toxin's binding subunit (RTB).
PLoS One.
Bai Y, Yang J, Eisele LE, Underwood AJ, Koestler BJ, Waters CM, Metzger DW, Bai G.
Two DHH subfamily 1 proteins in Streptococcus pneumoniae possess cyclic di-AMP phosphodiesterase activity and affect bacterial growth and virulence.
J Bacteriol.
Callahan B, Nguyen K, Collins A, Valdes K, Caplow M, Crossman DK, Steyn AJC, Eisele L, Derbyshire KM.
Conservation of structure and protein-protein interactions mediated by the secreted mycobacterial proteins EsxA, EsxB, and EspA.
Journal of Bacteriology.
Eisele LE, Chave KJ, Lehning AC, Ryan TJ.
Characterization of Human gamma-glutamyl hydrolase in solution demonstrates that the enzyme is a non-dissociating homodimer.
Biochem. Biophys. Acta.
Bennett MT, Rodgers MT, Hebert AS, Ruslander LE, Eisele L, Drohat AC.
Specificity of human thymine DNA glycosylase depends on N-glycosidic bond stability.
J Am Chem Soc.