Olympus SZX12 (Stereoscope)

Dissecting microscope for low magnification observation of specimens with fluorescence capabilities (UV, RFP, GFP) and a Nikon DS-Fi3 color CMOS camera.

Nikon Eclipse 50i

An upright clinical line microscope offering transmitted and fluorescent capabilities with a Nikon DS-Fi3 color CMOS camera.  Available objectives: 1x/0.04; 4x/0.13; 10x/0.25 Ph1 DL; 20x/0.75 Ph2 DM; 40x/0.95 DIC M.

Nikon Ti2-E w/ environmental chamber (Widefield)

Motorized inverted fluorescence microscope equipped with a Lumencor AURA III Light Engine offering 375/30, 475/28, 555/28, 635/22, and 730/40 nm excitation wavelengths with DAPI, GFP, RFP, TxRed, and Quad filter cubes plus a pco.edge sCMOS 4.2 megapixel camera with 6.5µm pixel size.  Live cell imaging capability is provided with a Tokai Hit dark type warming box with temperature control.  Available objectives: 4x/0.13 PhL; 10x/0.3 Ph1; 20x/0.50 Ph1; 20x/0.75 Ph2; 40x/0.95 DIC; 60x/1.40 Oil DIC; Software:  NIS Elements Advanced Research version 6.10.01.

Nikon Ti2-E w/ iLas2 (Widefield, TIRF, FRAP)

Motorized inverted fluorescence microscope equipped with a Lumencor Spectra III epifluorescence LED-based light engine offering 390, 440, 475, 510, 555, 635, and 740 nm wavelengths with filter cubes for DAPI, CFP, GFP/FITC/Cy2, YFP, DSRed/TRITC/Cy3, Cy5, and Cy7 plus a Photometrics Prime BSI Express sCMOS 4.2 megapixel camera with 6.5µm pixel size.  Transmitted light modalities include brightfield, DIC, and Phase Contrast.  Additional iLas2 system provides TIRF and FRAP modules with 488/561/640nm (TIRF) and 405/488/561nm (FRAP) LASER lines.  Live cell imaging capability is provided with a Tokai Hit stage-top incubator with 5% CO2 and/or temperature control.  Available objectives: 10x/0.45 DIC; 20x/0.80 DIC; 40x/0.95 DIC; 40x/1.3 Oil DIC; 60x/1.42 Oil DIC; 100x/1.45 Oil DIC; 100x/1.45 Oil Ph3; 100x/1.49 Oil.  Software: NIS Elements Advanced Research version 6.10.01.

Leica DMi8 w/ Dragonfly Kit (Spinning Disk Confocal)

Motorized inverted fluorescence microscope with Dragonfly 200 high speed spinning disk confocal platform equipped with 405, 488, 561, and 637 nm excitation LASER lines with 445/46, 521/38, 594/43, and 698/77 emission filters plus a Zyla 4.2 PLUS 4.2 megapixel camera with 6.5µm pixel size.  Okolab Bold Line unit provides temperature, gas, and humidity control for live cell imaging with a cage incubator.  Available objectives:  10x/0.40, 20x/0.70 Imm, 20x/0.70, 40x/1.25 Oil, 63x/1.2 Water, 63x/1.4 Oil.  Software: Fusion version 2.3.0.44

Nikon AXR 2k Resonant Confocal w/ NSPARC (Point Scanning Confocal)

Motorized inverted confocal microscope equipped with 405, 488, 561, and 640 nm excitation LASER lines plus 4-channel hybrid GaAsP/PMT, NSPARC, and transmitted light detectors, and a Prime BSI sCMOS camera for continuous scanning sample navigation.  Live cell imaging capability is provided with a Tokai Hit stage-top incubation system with CO2 and temperature control.  Available objectives: 4x/0.20; 10x/0.45 DIC; 20x/0.80 DIC; 40x/0.95 DIC; 60x/1.42 Oil DIC; 60x/1.20 Water DIC.  Software: NIS Elements Advanced Research version 6.10.01.

Nikon Ti2-E w/ Abberior Stedycon (STED Superresolution)

Motorized inverted microscope equipped with Abberior STEDYCON module for confocal and multicolor STED imaging with 405, 488, 560, and 640 nm pulsed excitation LASER lines and a 775 nm pulsed STED LASER allows for four Confocal and two STED imaging channels.  Four single-photon-counting APD modules allow for extremely low dark counts to render the finest details and superior photon detection sensitivity (>62% typical).  Detection bands: 420-480, 505-550, 575-625, 650-700.  Capable of achieving lateral resolution of less than 40nm (typically 30nm) depending on dyes and objectives used.  Available objectives: 20x/0.75 multi-immersion DIC; 100x/1.45 Oil DIC; 100x/1.30 Oil Ph3.  Software: STEDYCON smart control browser-based software.

Image Analysis Workstations

Computer workstations are available for image processing.  Available software:  Autoquant, Image Pro, Imaris, NIS Elements, & Fiji.