Published on New York State Department of Health, Wadsworth Center (

Advanced Light Microscopy & Image Analysis Services Provided

For any of the services described below, please complete and submit the Service Request Form[1].

Tissue (100-1000X)

Most fluorescently labeled tissue slices (100µm uncleared and up to 500µm cleared) are best imaged with a confocal microscope. The confocal microscope removes most of the "out-of-focus" blur associated with imaging fluorescent specimens that are thicker than the depth of field of the objective lens.
Multi-channel: up to 3 biomolecules/fluorophores can be collected simultaneously with reflection microscopy. Z series can be combined with multiple biomolecules or fluorophores.

Cells (100-1000X)

  • Live (room temperature to 50ºC)
    • Transmitted light:
      • Phase contrast
      • Differential interference contrast (DIC)
      • Time-lapse
    • Fluorescence: Metal halide illumination
      • FRAP - example in gallery
      • Time lapse of one or more biomolecules or fluorophore 
      • Z series (can be combined with multi biomolecules or fluorophore)
  • Fixed (fluorescent or histological stains)
    • Multi-channel:
      • Up to 5 biomolecules/fluorophore
      • Z series can be combined with multi biomolecules or fluorophore
    • True color:
      • Absorbance stains
      • Bright immuno-fluorescence

Whole mounts

  • Confocal: object(s) <500 µm
    • Up to 3 biomolecules/fluorophores simultaneously with reflection microscopy
    • Z series (100µm uncleared and up to 500µm cleared)
    • Magnification (40 -1000X)
  • Dissecting scope: object(s) 300 µm to mm's
  • Up to 3 biomolecules/fluorophores with transmitted light mode
  • Magnification (10 -600X)
  • Extensive measurements tools


  • Opaque objects: small parts, tobacco, etc.
    • magnification (10 -1000X)
    • Extensive measurements tools
  • Phase objects: Crystals
    • Polarized light
    • Bright field