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3D-EM Group

Ribosome with mammalian mitochondrial initiation factor 2 Ryanodine receptor with ligands and sequence markers Various types of microtubules Topology of mitochondrial membranes Cryo-FIB preparation of muscle tissue Contrast enhancement by in-focus phase-plate imaging

The Wadsworth Center 3D-EM Group consists of Wadsworth researchers who develop and use the latest advances in electron microscopy and image processing to study cellular processes, disease mechanisms and microbial pathogens. The techniques pioneered at Wadsworth are used by scientists around the world, and images generated over the past 4 decades can be found in top journals, textbooks and encyclopedias.

Biological Research Areas

Ribosomes and the mechanism of protein translation

As the site of protein synthesis, the functioning of this macromolecule is relevant to a wide range of diseases and disorders. Read More >>

 

Functional states of the ryanodine receptor (RyR) and 3D structure of the triad junction

This macromolecule, also known as the calcium-release channel, mediates muscle contraction and is involved in disorders of both skeletal and cardiac muscle. Read More >>

 

Microtubule-based complexes and organelles

Microtubules are present in almost all cells, and are responsible for many forms of motility, as well as playing a key role in cell division. Read More >>

 

Mitochondria

This cellular organelle is responsible for energy production, and is sensitive to and indicative of many pathological states. Read More >>

Technological Development Areas

Single-particle cryo-EM

Individual projection images of many examples of a single ice-embedded macromolecule are combined to yield a high-resolution 3-D reconstruction. Read More >>

 

Cryo-electron tomography

Projection images of a unique, vitreously frozen subcellular feature are recorded over a range viewing angles and combined into a 3-D reconstruction. Read More >>

 

SPIDER and Web image-processing software

A suite of image-processing operations and protocols for creating 3-D reconstructions from electron microscope images, SPIDER and Web are used in about 100 laboratories worldwide. Read More >>

 

Cryo-focused-ion-beam (FIB) preparation of vitreous specimens for cryo-TEM

A new technique to create thin TEM samples from frozen, native-state biological material allows cryo-tomographic 3-D reconstruction from any type of cell or tissue. Read More >>

 

Phase-plate imaging for cryo-TEM

Phase plates facilitate TEM imaging of unstained biological specimens without an oscillating transfer function, greatly improving overall contrast and simplifying image analysis. . Read More >>

 

Time-resolved single-particle Cryo-TEM

Millisecond-range intermediates in macromolecular interactions can be captured for cryo-TEM study. Read More >>

 

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© 2014 Wadsworth Center, New York State Department of Health