Karen J. Chave
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Karen J. Chave, PhD

Director, Protein Expression and Biochemistry Cores
Research Scientist, Scientific Core
PhD: University of Surrey (1997)
Postdoctoral training: New York State Department of Health, Wadsworth Center

Research Interests

I am the Director of the Wadsworth Center Protein Expression Core. This core performs recombinant expression and purification of proteins in E. coli, insect and mammalian expression systems. I have over 30 years of experience in the field and specialize in finding conditions for expression of soluble protein in E. coli. I have expressed and purified over 170 proteins. 


I also direct the Wadsworth Center Biochemistry Core. This core provides instrumentation for the analysis of biomolecules and complexes. Core staff help investigators design experiments, collect, analyze and interpret data.


I am the section head for Blood pH and Gases, Clinical Chemistry, Endocrinology, and Therapeutic Substance monitoring/Quantitative Toxicology for the Clinical Laboratory Evaluation Program.  I review lab developed tests, Certificate of Qualification applications, and act as a subject matter expert.
 

Select Publications
Yates JL, Hunt DT, Kulas KE, Chave KJ, Styer L, Chakravarthi ST, Cai GY, Bermúdez-González MC, Kleiner G, Altman D, Srivastava K; PVI Study Group; Simon V, Feihel D, McGowan J, Hogrefe W, Noone P, Egan C, Slifka MK, Lee WT. Development of a novel serological assay for the detection of mpox infection in vaccinated populations. J Med Virol. 2023; Oct;95 (10): e29134. DOI: 10.1002/jmv.29134
Pubmed Web Address
Chave KJ. Gamma-glutamyl-Hydrolase. "Handbook of Proteolytic Enzymes". 2013; (3rd Edition, Academic Press): 2453-2458.
Eisele LE, Chave KJ, Lehning AC, Ryan TJ. Characterization of Human gamma-glutamyl hydrolase in solution demonstrates that the enzyme is a non-dissociating homodimer. Biochim. Biophys. Acta. 2006; 1764 (9): 1479-86.
Pubmed Web Address
Volk EL, Pankuch JJ, Chave KJ, Coward JK, Schneider E. A rapid assay for the quantitation of gamma-glutamyl hydrolase using a fluorogenic peptide as substrate. BioTechniques. 2003; 35 (5): 926-928.
Pubmed Web Address
Chave KJ, Ryan TJ, Chmura SE, Galivan J. Identification of single nucleotide polymorphisms in the human gamma-glutamyl hydrolase gene and characterization of promoter polymorphisms. Gene. 2003; 319 (NULL): 167-175.
Pubmed Web Address
Li H, Ryan TJ, Chave KJ, Van Roey P. Three-dimensional structure of human gamma-glutamyl hydrolase. A class I glatamine amidotransferase adapted for a complex substate. J Biol Chem. 2002; 277 (27): 24522-29.
Pubmed Web Address
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