Richard Cole

Richard Cole, M.A.

Director, Light Microscopy Core
Assistant Professor, School of Public Health, Biomedical Sciences
M.A., State University of New York at New Paltz, NY
(518) 474-7048

Research Interests

The Advanced Light Microscopy and Image Analysis (ALM&IA) Core is a resource for investigators within the Wadsworth Center as well as those from outside institutions who require advanced light microscopy (LM) and/or image analysis as part of their research programs.

Examples of these techniques are: 6D-wide field fluorescence microscopy, deconvolution, long-term imaging (transmitted and/or fluorescent) in a near homeostatic environment, and fluorescent recovery after photo bleaching (FRAP). There are also two state-of-the-art confocal microscopes (Leica SP5) in the core for imaging thicker samples. The facility supplies equipment, technical expertise and consumable supplies for various LM studies. Core personnel work along side investigators who may lack the technical expertise needed to develop imaging strategies and image analysis protocols needed to solve their research questions.

My research interests are in developing imaging/analysis solution to solve biological questions. In the past, I designed and built a combined laser microsurgery optical trapping workstation that allowed investigators to ablate part of or entire organelles within a living cell. Another imaging technology that I am developing is confocal multi-photon intra-vital imaging. The need to image in in-vivo is obvious. By using ultra-short (0.000000000001 second) laser pulses it is possible to image very deep, up to 0.5 mm with very little damage to the cells in the tissue. This technique is extremely powerful and we are currently studying microvessel angiogenesis in skin.

Select Publications
Brown C, Reilly A, Cole RW. A Quantitative Measure of Field Illumination. J Biomol Tech. 2015;
Ahlawat S, De Jesus M, Khare K, Cole RA, Mantis NJ. Three-dimensional reconstruction of murine Peyer's patches from immunostained cryosections. Microsc Microanal. 2014; 20 (1): 198-205.
Jonkman J, Brown CL, Cole RW. Quantitative Confocal Microscopy: Beyond a Pretty Picture. Methods Cell Biol. 2014; (123): 113-34.
Cole, R. W., Jinadasa, T. and Brown, C. M. Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control. Nature Protocols. 2011; 6 (12): 1929-41.
Full publication listing