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Services Provided

The Wadsworth Center Histopathology Core provides high-quality sectioning and staining of animal tissues to assist investigators in the determination of structural defects and physiological abnormalities. Investigators from many WC divisions use the core – from Infectious Disease to Genetic Disorders to Environmental Disease Protection.  

Investigators submit fixed tissues, which are then processed and paraffin-embedded prior to sectioning on a rotary microtome.  You can specify the plane of section, the number of sections per slide, and the number of slides of each type to be prepared. Charges are determined per slide. Services Offered Include:

  • Hematoxylin and Eosin (H&E) Staining: H&E is the standard stain for histopathological assessment of tissues. 
     
  • Periodic acid-Schiff (PAS) Staining: Stains glycogen, mucopolysaccharides and mucin red-purple; also useful for detecting fungal organisms in tissues.
     
  • Mucicarmine Staining: Stains epithelial mucin red; also useful in detecting cryptococcus that has a mucopolysaccharide coat.
     
  • Masson’s Trichrome Staining: Stains nuclei deep blue, skeletal and smooth muscles red, and collagen and mucin blue. 
     
  • Giemsa Staining: Differentiates cell types in blood smears and bone marrow specimens.  Also used to stain chromosomes, some fungi and pathogenic bacteria.
     
  • Amyloid Staining: Stains amyloid (fibrous protein aggregates) present in tissues.
     
  • Unstained Slides: For alternative lab-specific histochemical or immunohistochemical staining, slides of tissue sections may be returned to the investigator to be stained in the laboratory of origin.  Sections are collected on coated (Plus slides or ProbeOn+) slides.