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Flow Cytometer Quality Control

Daily flow cytometer quality control requirements:

  • Calibrate and standardize according to the manufacturer's recommendation (software and bead product). 
    • Cellular Immunology Standard CILF-S11, CINM-S6, CIML-S7
  • Stain and analyze a normal blood and/or validated commercial control(s) for Non-malignant Leukocyte Immunophenotyping. 
    • Cellular Immunology Standard CINM-S11

Monthly flow cytometer quality control requirements:

  • Check fluorescence/PMT linearity. 
    • Cellular Immunology Standard CILF-S12, CINM-S7, CIML-S8
  • Stain and analyze a normal blood or validated commercial control for Malignant Leukocyte analysis. 
    • Cellular Immunology Standard CINM-S9

As the manufacturer recommends:

  • Clean system fluidics.
  • Perform preventive maintenance.
  • Verify optical alignment on fixed optical rail flow cytometers.

If an instrument is moved:

  • Verify optical alignment.
  • Calibrate and standardize according to the manufacturer's recommendations for software and bead product. 
    • Cellular Immunology Standard CILF-S11, CINM-S6, CIML-S7
  • Check fluorescence/PMT linearity. 
    • Cellular Immunology Standard CILF-S12, CINM-S7, CIML-S8
  • Stain and analyze a normal blood and/or validated commercial control(s).
  • These guidelines should also be followed when evaluating instrument performance following an instrument repair. Please refer to the Cellular Immunology Checklist on the Clinical Laboratory Evaluation Program method approval webpage for instrument validation requirements.