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Protein Expression

The Wadsworth Center Protein Expression Core is available to help generate the protein of interest and is able to assist in many aspects of protein expression and/or purification.

Services:

  • Construction of expression clones
  • Screening for soluble protein production
  • Expression of recombinant proteins in E.coli
  • Purification of proteins (soluble and insoluble)
  • Antibody production from a verified hybridoma
  • Monoclonal and polyclonal antibody purification
  • Endotoxin reduction of purified proteins
  • Insect cell expression mediated by baculovirus
  • Adenovirus generation and amplification
  • Lentivirus generation and amplification
  • Custom affinity column synthesis (attach an antibody or peptide to resin)
  • Consultation and troubleshooting all aspects of protein expression and purification

Facilities:

  • Shaker incubators capable of 4oC to 40oC incubation
  • Akta FPLC chromatography system
  • 2x Akta Prime chromatography systems (one at 4oC)
  • Bio-Rad DuoFlow chromatography with maximizer
  • Many gel filtration and ion exchange columns and resins
  • Amicon protein concentration stirred cells with multiple MW cutoff filters
  • Bio-Rad ChemiDoc XRS+ gel documentation and chemiluminescence detection system
  • Molecular Devices Spectramax M2 absorbance and fluorescence multiplate reader with temperature control
  • Beckman Spectrophotometer
  • Eppendorf EpMotion 5070 pipetting robot
  • Cell culture/molecular biology/SDS-PAGE equipment

In order to request service from the Protein Expression Core, please contact Dr. Karen Chave, Director.

“We spent more than 4 months trying to optimize conditions for our prokaryotic expression prior to using the Protein Expression Core services. The Core’s staff used their expertise to provide milligram quantities of purified protein within a week and was well worth the expense.”  -Bruce Herron, Ph.D. New York State Department of Health, Wadsworth Center