The Applied Genomic Technologies Core provides next-generation sequencing, Sanger sequencing, genotyping and DNA extraction, quantitation and purification services. Next-generation sequencing services are provided using MiSeq (Illumina) and the Ion Torrent PGM (Life Technologies) sequencers. Services are available for amplicon re-sequencing, viral or microbial whole genome sequencing and 16S microbiome sequencing. Sanger DNA Sequencing can be performed on DNA templates such as PCR products and plasmids using ABI 3730xl and ABI3130xl instruments.
Genotyping services include a variety of approaches for genotyping, including DNA purification and PCR troubleshooting/optimization services. The types of genotyping analyses offered include fragment analysis, Taq-Man-based single nucleotide polymorphism (SNP) and gene copy number variation (gene dosage) analysis using multiplex ligation-dependent probe amplification (MLPA). Human cell line verification by short tandem repeat (STR) testing is also available.
For general inquiries and to request service, please contact the genomics core staff.