The Media and Tissue Culture Core supports all Wadsworth Center labs including clinical, environmental, and research labs. The Tissue Culture Core maintains a repository of many hundreds of cell lines available for use in research and/or public health. These cell cultures are used for the propagation of viruses and expression of specialized biological products, such as hormones and other types of proteins. The Tissue Culture Media and Media cores maintain a large stock of dehydrates, chemicals, and reagents used for tissue culture, bacteriology, mycology, parasitology, and virology media preparation. The core follows strict Quality Control (QC) procedures, including several ISO protocols, to ensure quality cells and media are prepared.

Services

Tissue Culture

  • Cell culture defrosting, maintenance, and expansion as well as freezing and storage (cryopreservation).
  • Accommodations for a wide variety of adherent and suspension mammalian and insect cell lines.
  • Cell harvest volumes from small scale (mL) to large scale (L).
  • Variety of tissue culture treated vessels (T flasks, roller bottles, etc.) for use. Tissue Culture Media
  • Preparation of completed media such as DMEM, EMEM, RPMI-1640, etc. with added supplements such as sodium bicarbonate, fetal bovine serum (FBS), Non-Essential Amino Acids (NEAA), sodium pyruvate and several antibiotics.
  • Preparation of reagents including Calcium-Magnesium free Dulbecco’s PBS and Trypsin-EDTA

Media

  • Preparation of simple to complex media, agar, reagents and buffers for laboratory work.
  • Large scale sterile filtration and vial dispensing of reagents.
  • Hard good sterilization (pipettes, microcentrifuge tubes, etc.).

Facilities

Tissue Culture

  • 3 - Thermo-Scientific Reach-in CO2 incubators with capacity for 36 roller bottles
  • 4 - Thermo-Scientific Forma Steri-Cyle CO2 Incubators
  • Thermo-Scientific Cryo Plus Liquid Nitrogen Storage (36,000 sample capacity)
  • Carl Zeiss Invertoskop Inverted Microscope
  • Thermo-Scientific Invitrogen Countess II Automated Cell Counter
  • Lonza Lucetta 2 Luminometer for Mycoplasma testing  

Tissue Culture Media and Media

  • 5 - Getinge 533LS Autoclave Clean Steam Sterilizers
  • 2 - Siemens Ultrapure “Millipore” Water Systems (Produces up to 18.2 megaohm water)
  • 7 - Analytical and General Balances (Accurate Weighing from 0.1 mg to 4100 grams)
  • 2 - Labconco Powder Containment Hoods

Staff

  • Experienced staff who are cross trained in multiple areas of the lab
  • Prepare >3000 media requests annually, including over 145,000 Petri dish plates/year
  • Prepare >14,000 liters of liquid media annually
  • Seed > 3,500 flasks annually

 

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