Tissue Culture

The Center maintains a repository of many hundreds of cell lines. Cell cultures are used for the propagation of viruses and expression of specialized biological products, such as hormones and other types of proteins. The Tissue Culture and Media core also assists in the preparation of certain types of proficiency test samples for other groups.

Services

• Cell culture; thawing, maintenance, expansion, as well as freezing and storage
• Accommodations for a wide range of cells from mammalian and avian to arthropod and hybridoma lines
• Cell harvest volumes from small scale (mL) to large scale (Liters)
• Variety of culture vessels
• Mycoplasma testing and eradication
• Human cell line authentication
• Preparation of specialized media and buffers
• Large scale sterile filtration and vial dispensing of reagents

Facilities

• 3-Thermo Scientific Reach in CO₂ Incubators, with capacity for 36 roller bottles
• 1-Thermo Scientific Environmental Chamber
• 2-Thermo Scientific Forma Steri-Cycle CO₂ Incubators
• Olympus IX71 inverted microscope equipped with DAPI and FITC filter sets
• Thermo Scientific Cryo Plus Liquid Nitrogen storage; 36,000 sample capacity
• MVE XLC-1110 Liquid Nitrogen storage; 25,000 sample capacity
• 1-12x12 Walk in cold room for media storage
• Clestra Cleanroom