Research in the Wade laboratory focuses on gene regulation and CRISPR-Cas immunity in a range of bacterial species.

Specific topics of interest include: 

  1. Regulation of transcription initiation and termination in Escherichia coli
  2. Regulation of virulence gene transcription in Salmonella enterica and Yersinia pestis 
  3. Mechanisms of CRISPR-Cas immunity in E. coli and S. enterica
  4. Translational regulation and small protein function in mycobacteria (in collaboration with the Derbyshire/Gray Laboratory)

Our research relies heavily on next-generation sequencing approaches for genome-scale and high-throughput analysis of gene regulation and CRISPR-Cas function.